HPLC Column, RP Phenyl, Wide Pore, 5um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP Phenyl™ Wide Pore HPLC Columns are designed for reversed-phase separations involving larger molecules that benefit from aromatic selectivity and enhanced pore accessibility. Manufactured using ultra-high purity silica bonded with a three-carbon linker and terminal phenyl functionality, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. The 300 Å wide pore structure improves analyte access to the stationary phase, making this column an excellent alternative for USP L11 methods involving larger compounds and for new method development.
68524-25P-4-3M is packed with 5 µm RP Phenyl™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 250 mm format. The wide pore structure improves mass transfer for larger molecules, including peptides and complex natural products, while the 5 µm particle size provides excellent chromatographic efficiency with moderate operating pressures. The extended 250 mm column length delivers maximum resolution and peak capacity for demanding separations requiring enhanced analyte discrimination.
The 4.0 mm ID x 250 mm format is well suited for reversed-phase separations of large positional isomers, peptides, natural products, and related compounds. Typical flow rates are approximately 0.8-1.2 mL/min depending on mobile phase composition and application requirements. This format is particularly valuable when maximum chromatographic resolution is required for complex samples and method development.
Key Benefits
RP Phenyl™ Wide Pore stationary phase
USP L11 equivalent chemistry
Ultra-high purity silica support
Fully double end-capped
Phenyl bonded phase
300 Å pore size
Suitable for reversed-phase HPLC
Enhanced aromatic selectivity
Improved accessibility for larger molecules
Maximum resolution and peak capacity
Typical Applications
Large positional isomer separations
Peptide analysis
Natural product analysis
Biomolecule separations
Pharmaceutical method development
Reversed-phase HPLC
Large molecule analysis
Aromatic compound separations
Research and development
Method optimization
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl™ Wide Pore
Chromatography Mode: Reversed Phase
USP Classification: L11
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Recommended Flow Rate: 0.8-1.2 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Suitable for reversed-phase chromatography
USP L11 equivalent chemistry
Fully double end-capped stationary phase
Wide pore design improves accessibility for larger molecules
Excellent for peptides, natural products, and large aromatic compounds
Useful for separation of large positional isomers and structurally related analytes
250 mm length provides maximum resolution and peak capacity
Ideal for demanding method development and complex sample analyses
Compatible with most standard HPLC systems
This is a Special Order Item