Catalog Number - 68524-P21-50-3M

Cogent RP Phenyl™ Wide Pore Preparative HPLC Columns are designed for high-capacity purification, preparative separations, and process development involving larger aromatic compounds and structurally related analytes. Manufactured using ultra-high purity silica bonded with a three-carbon linker and terminal Phenyl functionality, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. The 300Å wide pore structure improves accessibility for larger molecules while maintaining the aromatic selectivity characteristic of RP Phenyl™ chemistry.

68524-P21-50-3M is packed with 5 µm RP Phenyl™ Wide Pore particles featuring a 300Å pore size in a 21.2 mm ID x 50 mm preparative format. The 5 µm particle size performs well at preparative flow rates while maintaining moderate operating backpressure. The large internal diameter provides substantial sample loading capacity, while the shorter column length helps minimize run times and solvent consumption for rapid preparative purification and method scale-up.

The 21.2 mm ID x 50 mm format is particularly well suited for purification of larger positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, peptides, and related aromatic compounds. Typical operating flow rates are approximately 15–25 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative HPLC system capable of supporting these flow rates and sample loads is required.

Key Benefits

• RP Phenyl™ Wide Pore stationary phase

• USP L11 equivalent chemistry

• Ultra-high purity silica support

• Fully double end-capped

• Phenyl bonded phase

• 300Å pore size

• Suitable for reversed-phase chromatography

• Enhanced aromatic selectivity

• Improved accessibility for larger molecules

• High sample loading capacity

Typical Applications

• Preparative purification

• Large positional isomer separations

• Tocopherol purification

• Flavonoid purification

• Polynuclear aromatic hydrocarbon separations

• Nitro-aromatic compound purification

• Active pharmaceutical ingredient (API) purification

• Natural product isolation

• Peptide purification

• Method scale-up and process development

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: RP Phenyl™ Wide Pore

• Chromatography Mode: Reversed Phase

• USP Classification: L11

• Particle Size: 5 µm

• Pore Size: 300Å

• Column Dimensions: 21.2 mm ID x 50 mm

• Recommended Flow Rate: 15–25 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

 Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• USP L11 equivalent chemistry

• Fully double end-capped stationary phase

• Wide pore design improves accessibility for larger molecules

• Excellent for purification of peptides, natural products, APIs, and other large aromatic compounds

• High loading capacity for preparative applications

• Typical operating range is approximately 15–25 mL/min

• Requires a specialized preparative HPLC system

• Short 50 mm length helps reduce purification times and solvent consumption

• Well suited for purification, fraction collection, and analytical-to-preparative method scale-up

• This is a Special Order Item