Catalog Number - 72030-05P

The 72030‑05P Cogent C30™ HPLC Column is designed for shape‑selective separation of long‑chain hydrophobic compounds, providing unique resolution of positional and geometric isomers that are difficult to separate with conventional C18 phases.

This column features a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), delivering stronger hydrophobic interaction and a distinct selectivity profile under reversed‑phase conditions.

With a 200 Å pore size, it is well suited for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent chromatographic performance.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a reliable alternative for USP L62 methods and equivalent workflows

50 mm Length – Fast Screening & Preparative Scouting

The 50 mm length is optimized for rapid separations and method scouting, particularly useful when developing conditions prior to scale‑up.

• Short run times and fast method evaluation

• Efficient for selectivity testing and screening

• Reduced solvent usage

✅ Use when developing methods intended for semi‑prep or preparative scale

Flow Rate & System Requirements (4.6 mm ID, 10 µm)

⚠️ Compatible with standard HPLC systems, with lower backpressure compared to smaller particles

• Typical flow rate range: ~0.8–2.0 mL/min

• Can support higher flow rates due to larger particle size

• Lower system pressure vs 3 µm and 5 µm columns

• Standard analytical detector compatibility

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond traditional C18 capability

Effective for Larger Molecules

• 200 Å pore supports complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and reproducibility issues

• Maintains consistent chromatographic performance

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent column performance

• Reliable reproducibility across methods

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• Method development and preparative scouting

Important Usage Notes

• Designed for standard HPLC systems (4.6 mm ID)

• Not end‑capped (intentional for selectivity differences)

• Lower backpressure vs smaller particle sizes

• Ideal for method development and scale‑up workflows