Catalog Number - 72030-10P

The 72030‑10P Cogent C30™ HPLC Column is designed for shape‑selective separation of long‑chain hydrophobic compounds, delivering reliable resolution of positional and geometric isomers that are difficult to separate using conventional C18 phases.

This column features a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and a distinct selectivity profile that enables separation of structurally similar compounds under reversed‑phase conditions.

With a 200 Å pore size, it is well suited for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent chromatographic performance.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a dependable alternative for USP L62 methods and similar workflows

100 mm Length – Increased Resolution for Method Development

The 100 mm length offers a balance between speed and resolution, providing improved separation over short screening columns while maintaining efficient run times.

• Better separation than 50 mm columns

• Improved resolution of closely related isomers

• Increased peak capacity for complex samples

✅ Use when greater resolution is needed before scaling to preparative formats

Flow Rate & System Requirements (4.6 mm ID, 10 µm)

⚠️ Compatible with standard HPLC systems, with lower backpressure compared to smaller particle sizes

• Typical flow rate range: ~0.8–2.0 mL/min

• Supports higher flow rates due to 10 µm particles

• Reduced system pressure vs 3 µm and 5 µm columns

• Standard analytical detector compatibility

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond traditional C18 capability

Effective for Larger Molecules

• 200 Å pore supports complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and reproducibility issues

• Maintains consistent, robust operation

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent column‑to‑column performance

• Reliable chromatographic reproducibility

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• Method development and preparative scouting