Catalog Number - 72030-15P

The 72030‑15P Cogent C30™ HPLC Column is designed for shape‑selective separation of long‑chain hydrophobic compounds, delivering reliable resolution of positional and geometric isomers that are often difficult to separate with standard C18 columns.

This column uses a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing enhanced hydrophobic interaction and a unique selectivity profile that enables separation of structurally similar compounds under reversed‑phase conditions.

With a 200 Å pore size, it is particularly effective for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent chromatographic performance.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a reliable alternative for USP L62 methods and similar analytical workflows

150 mm Length – High Resolution Before Scale‑Up

The 150 mm length increases resolving power over shorter columns, making it ideal for improving separations prior to moving to preparative scale.

• Higher resolution than 50 mm and 100 mm columns

• Improved separation of positional and geometric isomers

• Increased peak capacity for complex mixtures

✅ Use when greater resolution is required before preparative scaling or purification workflows

Flow Rate & System Requirements (4.6 mm ID, 10 µm)

⚠️ Compatible with standard HPLC systems, with lower backpressure than smaller particle sizes

• Typical flow rate range: ~0.8–2.0 mL/min

• Supports higher flow rates due to 10 µm particle size

• Lower system pressure compared to 3 µm and 5 µm columns

• Standard analytical detector compatibility

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond traditional C18 capability

Effective for Larger Molecules

• 200 Å pore supports complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and reproducibility issues

• Maintains consistent chromatographic performance

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent performance across runs

• Reliable chromatographic reproducibility

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• Method development and preparative scouting

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Not end‑capped (intentional for selectivity differences)

• Lower backpressure vs smaller particle sizes

• Ideal for method development and scale‑up workflows