Catalog Number - 72030-25P

The 72030‑25P Cogent C30™ HPLC Column is designed for maximum‑resolution, shape‑selective separation of long‑chain hydrophobic compounds, delivering reliable performance for positional and geometric isomers that are difficult to resolve with conventional C18 phases.

This column utilizes a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing enhanced hydrophobic interaction and a unique selectivity profile that enables accurate separation of structurally similar compounds under reversed‑phase conditions.

The 200 Å pore size supports larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent retention and chromatographic behavior.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a dependable alternative for USP L62 methods and equivalent workflows

250 mm Length – Maximum Resolution Before Scale‑Up

The 250 mm length provides the highest resolving power in the 10 µm C30 analytical range, making it ideal for difficult separations and final method optimization prior to preparative scaling.

• Maximum separation of positional and geometric isomers

• Highest peak capacity for complex mixtures

• Improved purity and resolution of closely eluting compounds

✅ Use when 150 mm columns do not achieve required resolution or separation quality

Flow Rate & System Requirements (4.6 mm ID, 10 µm)

⚠️ Compatible with standard HPLC systems, with significantly lower backpressure than smaller particle sizes

• Typical flow rate range: ~0.8–2.0 mL/min

• Supports higher flow rates due to 10 µm particles

• Lower pressure vs 3 µm and 5 µm columns

• Monitor pressure when using longer column length and viscous solvents

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Maximum Shape Selectivity

• Resolves positional and geometric isomers

• Enables separations beyond traditional C18 columns

Effective for Larger Molecules

• 200 Å pore enhances interaction with complex analytes

• Ideal for carotenoids, vitamins, and peptides

Robust Alternative to Normal Phase

• Avoids instability and variability issues

• Maintains consistent reversed‑phase performance

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent chromatographic performance

• Reliable reproducibility across runs

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• Method development and pre‑preparative optimization

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Not end‑capped (intentional for selectivity differences)

• Lower backpressure vs smaller particle sizes

• Ideal for maximum‑resolution method development and scale‑up workflows