HPLC Column, C30, 3um, 4.6mm ID x 50mm Length, 200A. Cogent Brand. 1 EA.
Additional Info:
The 72330‑05P Cogent C30™ HPLC Column is designed for enhanced shape selectivity and separation of long‑chain hydrophobic compounds, delivering superior resolution for positional and geometric isomers compared to standard C18 phases.
Manufactured using a 30‑carbon bonded phase on high‑purity silica (non‑end capped), this stationary phase provides stronger hydrophobic interaction and a different selectivity profile than traditional reversed‑phase columns. This makes it especially useful when conventional C18 methods cannot resolve structurally similar compounds.
The 200 Å pore size further expands its applicability to larger molecules, including carotenoids, vitamins, peptides, and some proteins.
50 mm Length – Fast Screening & Method Development
At 50 mm, this column is optimized for fast separations and efficient method scouting, ideal for evaluating selectivity before moving to longer columns.
Short run times and rapid gradients
Efficient screening for complex mixtures
Reduced solvent usage
✅ Use when fast method development and selectivity evaluation are priorities
Flow Rate & System Requirements (4.6 mm ID, 3 µm)
⚠️ Compatible with standard HPLC systems, but higher pressure than 5 µm columns should be expected
Typical flow rate range: ~0.8–1.5 mL/min
Optimal starting range: ~1.0–1.2 mL/min
Standard analytical detector compatible
Monitor system pressure due to 3 µm particle size
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose C30 for Hydrophobic Separations
Shape Selectivity for Isomers
Resolves positional and geometric isomers
Superior to C18 for structurally similar compounds
Ideal for Large Hydrophobic Molecules
200 Å pore supports larger analytes
Effective for carotenoids, vitamins, and peptides
Alternative to Normal Phase Methods
Avoids instability and variability of normal phase
Works under reversed‑phase conditions
Cogent C30™ Advantages
Extended C30 Ligand
Stronger hydrophobic interaction vs C18
Unique selectivity profile
High Purity Silica
Consistent performance and reproducibility
Reliable chromatographic behavior
Efficient Analytical Format (4.6 mm)
Compatible with standard HPLC systems
Ideal for method development and QC workflows
Typical Applications
Carotenoid and vitamin analysis
Isomer and positional compound separation
Peptide and large molecule analysis
Hydrophobic compound profiling
Method development and optimization
Important Usage Notes
Designed for standard analytical HPLC systems (4.6 mm ID)
Not end‑capped (intentional for selectivity)
Higher backpressure vs 5 µm columns
Ideal for shape‑selective separations where C18 is insufficient