Catalog Number - 72330-05P-2

The 72330‑05P‑2 Cogent C30™ HPLC Column is designed for enhanced shape selectivity and separation of long‑chain hydrophobic compounds, delivering improved resolution for positional isomers and structurally similar analytes.

Manufactured using a 30‑carbon bonded phase on high‑purity silica (non‑end capped), this column provides different selectivity compared to traditional C18 phases. The extended ligand length increases hydrophobic interaction and enables separation mechanisms not achievable with standard reversed‑phase columns.

The 200 Å pore size makes this column well suited for larger molecules, including carotenoids, vitamins, peptides, and some proteins.

50 mm Length – Fast Screening & Method Development

The 50 mm length is optimized for rapid separations, making it ideal for screening and early method development work.

• Short run times and fast gradients

• Efficient for method scouting

• Reduced solvent consumption

✅ Use when speed and method development flexibility are priorities

Flow Rate & System Requirements (2.1 mm ID, 3 µm)

⚠️ Requires low‑flow HPLC or LC‑MS system capability

• Typical flow rate range: ~0.2–0.3 mL/min

• Requires precise low‑flow pump control

• Compatible with low‑volume LC‑MS detector systems

• Higher backpressure vs 5 µm columns

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Enhanced Shape Selectivity

• Separates positional and geometric isomers

• Provides resolution not achievable with C18

Ideal for Large Hydrophobic Molecules

• Effective for carotenoids, vitamins, and peptides

• 200 Å pore supports larger analyte access

Alternative to Normal Phase

• Avoids common issues with normal phase chromatography

• Works under reversed‑phase conditions

Cogent C30™ Advantages

Extended Carbon Chain (C30)

• Stronger hydrophobic interaction than C18

• Improved selectivity for long‑chain compounds

High Purity Silica

• Consistent performance and reproducibility

• Reliable peak shape across runs

LC‑MS Compatibility

• Narrow 2.1 mm format optimized for sensitivity

• Ideal for low‑flow analytical systems

Typical Applications

• Carotenoid and vitamin analysis

• Peptide and large molecule separations

• Isomer and positional variant separation

• Hydrophobic compound profiling

• LC‑MS analytical workflows

Important Usage Notes

• Designed for low‑flow LC‑MS systems (~0.2–0.3 mL/min)

• Not end‑capped (intentional for selectivity differences)

• Higher backpressure vs 5 µm columns

• Ideal for shape‑selective separations and method development