HPLC Column, C30, 3um, 4.6mm ID x 100mm Length, 200A. Cogent Brand. 1 EA.
Additional Info:
The 72330‑10P Cogent C30™ HPLC Column is designed for enhanced shape selectivity and improved separation of long‑chain hydrophobic compounds, delivering superior resolution of positional and geometric isomers compared to standard C18 phases.
Manufactured with a 30‑carbon bonded phase on high‑purity silica (non‑end capped), this column provides stronger hydrophobic interaction and a distinct selectivity profile that enables separations not achievable with traditional reversed‑phase columns.
With a 200 Å pore size, it is especially effective for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining excellent chromatographic performance.
100 mm Length – Increased Resolution for Analytical Methods
The 100 mm length offers a balance between speed and resolution, allowing improved separation without the longer run times of 150 mm or 250 mm columns.
Better resolution than 50 mm screening columns
Improved separation of closely related isomers
Increased peak capacity for complex samples
✅ Use when additional resolving power is required beyond fast screening methods
Flow Rate & System Requirements (4.6 mm ID, 3 µm)
⚠️ Compatible with standard HPLC systems, but higher pressure than 5 µm columns should be expected
Typical flow rate range: ~0.8–1.5 mL/min
Optimal starting range: ~1.0–1.2 mL/min
Standard analytical detector compatible
Monitor system pressure due to smaller particle size
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose C30 for Hydrophobic Separations
Shape Selectivity for Isomers
Resolves positional and geometric isomers
Provides separation beyond traditional C18 capability
Optimized for Larger Molecules
200 Å pore improves accessibility for complex analytes
Ideal for carotenoids, vitamins, and peptides
Reliable Alternative to Normal Phase
Eliminates instability and variability of normal phase methods
Maintains reproducible reversed‑phase operation
Cogent C30™ Advantages
Extended C30 Ligand
Stronger hydrophobic interaction than C18
Unique selectivity for long‑chain compounds
High Purity Silica
Consistent column‑to‑column performance
Reliable and reproducible results
Analytical 4.6 mm Format
Compatible with standard HPLC systems
Ideal for method development and routine workflows
Typical Applications
Carotenoid and vitamin analysis
Isomer and positional compound separation
Peptide and larger molecule separations
Hydrophobic compound profiling
Method development and optimization
Important Usage Notes
Designed for standard analytical HPLC systems (4.6 mm ID)
Not end‑capped (intentional for enhanced selectivity)
Higher backpressure vs 5 µm columns
Ideal for shape‑selective separations where C18 is insufficient