HPLC Column, C30, 3um, 2.1mm ID x 100mm Length, 200A. Cogent Brand. 1 EA.
Additional Info:
The 72330‑10P‑2 Cogent C30™ HPLC Column is designed for enhanced shape selectivity and higher‑resolution separation of long‑chain hydrophobic compounds, delivering improved performance for positional and geometric isomers compared to traditional C18 phases.
This column uses a 30‑carbon bonded phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and a different selectivity profile that enables separations not achievable with standard reversed‑phase columns.
The 200 Å pore size allows efficient interaction with larger molecules, making it well suited for carotenoids, vitamins, peptides, and some proteins.
100 mm Length – Increased Resolution for LC‑MS Methods
The 100 mm length offers greater resolving power than 50 mm columns, improving separation while maintaining reasonable run times in LC‑MS workflows.
Better separation of closely related isomers
Increased peak capacity for complex samples
Ideal for method refinement after initial screening
✅ Use when 50 mm columns do not provide sufficient resolution
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
⚠️ Requires low‑flow HPLC or LC‑MS system capability
Typical flow rate range: ~0.2–0.3 mL/min
Requires precise, stable low‑flow pump performance
Compatible with low‑volume LC‑MS detector flow cells
Higher backpressure than 5 µm columns
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose C30 for Hydrophobic Separations
Shape Selectivity for Isomers
Resolves positional and geometric isomers
Provides separation beyond traditional C18 capability
Optimized for Large Hydrophobic Molecules
200 Å pore improves access for larger analytes
Effective for carotenoids, vitamins, peptides
Reversed‑Phase Alternative to Normal Phase
Eliminates instability issues of normal phase methods
Maintains reproducibility in standard HPLC/LC‑MS systems
Cogent C30™ Advantages
Extended C30 Ligand
Stronger hydrophobic interaction than C18
Unique selectivity for long‑chain compounds
High Purity Silica
Consistent column performance
Reliable reproducibility across methods
LC‑MS Optimized Format (2.1 mm ID)
Low‑flow compatible for improved sensitivity
Ideal for trace‑level and complex analyses
Typical Applications
Carotenoid and vitamin analysis
Isomer and positional compound separations
Peptide and larger molecule separations
Hydrophobic compound profiling
LC‑MS method development and optimization
Important Usage Notes
Designed for low‑flow LC‑MS systems (~0.2–0.3 mL/min)
Not end‑capped (intentional for selectivity differences)
Higher backpressure vs 5 µm columns
Ideal for shape‑selective separations where C18 is insufficient