Catalog Number - 72330-15P

The 72330‑15P Cogent C30™ HPLC Column is designed for high‑resolution, shape‑selective separation of long‑chain hydrophobic compounds, delivering superior performance for positional and geometric isomers where traditional C18 phases are often insufficient.

Manufactured using a 30‑carbon bonded phase on high‑purity silica (non‑end capped), this column provides stronger hydrophobic interaction and a distinct selectivity profile that enables separation of closely related structures under reversed‑phase conditions.

The 200 Å pore size expands its applicability to larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining excellent peak shape and retention.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a reliable alternative for USP L62 methods and equivalent workflows

150 mm Length – High Resolution for Analytical Work

The 150 mm length provides greater resolving power than shorter columns, allowing improved separation of closely related compounds and isomers.

• Enhanced resolution vs 50 mm and 100 mm columns

• Improved separation of positional and geometric isomers

• Higher peak capacity for complex samples

✅ Use when maximum resolution is needed in analytical HPLC workflows

Flow Rate & System Requirements (4.6 mm ID, 3 µm)

⚠️ Compatible with standard HPLC systems, but higher pressure than 5 µm columns must be considered

• Typical flow rate range: ~0.8–1.5 mL/min

• Recommended starting range: ~1.0–1.2 mL/min

• Standard analytical detector compatibility

• Monitor system pressure, especially with viscous mobile phases and longer runs

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separations not achievable with C18

Optimized for Larger Molecules

• 200 Å pore allows better access for larger analytes

• Ideal for carotenoids, vitamins, and peptides

Alternative to Normal Phase Methods

• Avoids instability and reproducibility issues of normal phase

• Maintains reversed‑phase method robustness

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent performance and reproducibility

• Reliable chromatographic behavior across runs

Method Transfer Compatibility

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and larger molecule separations

• Hydrophobic compound profiling

• Method development and optimization

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Not end‑capped (intentional for enhanced selectivity)

• Higher backpressure vs 5 µm columns

• Ideal for high‑resolution, shape‑selective separations