HPLC Column, C30, 3um, 2.1mm ID x 150mm Length, 200A. Cogent Brand. 1 EA.
Additional Info:
The 72330‑15P‑2 Cogent C30™ HPLC Column is designed for maximum‑resolution separation of long‑chain hydrophobic compounds, delivering exceptional performance for positional and geometric isomers where standard C18 phases fall short.
Manufactured with a 30‑carbon bonded phase on high‑purity silica (non‑end capped), this stationary phase provides stronger hydrophobic interaction and unique selectivity, enabling separations not achievable with traditional reversed‑phase columns.
The 200 Å pore size further enhances performance with larger molecules, making this column especially effective for carotenoids, vitamins, peptides, and some proteins where shape selectivity is critical.
✅ Comparable selectivity and application performance to YMC® C30 columns, making it an excellent alternative for L62 methods and equivalent workflows
150 mm Length – Maximum Resolution for LC‑MS
The 150 mm length delivers the highest resolving power in the 2.1 mm C30 format, making it ideal for difficult separations and trace analysis.
Superior separation of closely related isomers
Increased peak capacity for complex samples
Improved resolution for trace‑level components
✅ Use when 100 mm columns do not provide sufficient resolution
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
⚠️ Requires low‑flow HPLC or LC‑MS system capability
Typical flow rate range: ~0.2–0.3 mL/min
Requires precise and stable low‑flow pump control
Compatible with low‑volume LC‑MS detectors
Higher backpressure than 5 µm columns
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose C30 for Hydrophobic Separations
Maximum Shape Selectivity
Resolves positional and geometric isomers
Provides separation beyond C18 capability
Optimized for Larger Molecules
200 Å pore enables interaction with complex compounds
Ideal for carotenoids, vitamins, and peptides
Proven Alternative to Normal Phase
Avoids instability of normal phase methods
Maintains reproducible reversed‑phase operation
Cogent C30™ Advantages
Extended C30 Ligand
Stronger hydrophobic interaction vs C18
Enables unique isomer selectivity
High Purity Silica
Consistent performance and reproducibility
Reliable chromatography across methods
Equivalent Method Transfer Option
Comparable to YMC® C30 column chemistry and applications
Suitable for transferring L62 methods without major redevelopment
Typical Applications
Carotenoid and vitamin analysis
Isomer and positional compound separations
Peptide and large molecule separations
Hydrophobic compound profiling
LC‑MS method development and validation
Important Usage Notes
Designed for low‑flow LC‑MS systems (~0.2–0.3 mL/min)
Not end‑capped (intentional for enhanced selectivity)
Higher backpressure vs 5 µm columns
Ideal for maximum‑resolution, shape‑selective separations