HPLC Column, C30, 3um, 4.6mm ID x 250mm Length, 200A. Cogent Brand. 1 EA.
Additional Info:
The 72330‑25P Cogent C30™ HPLC Column is engineered for maximum‑resolution, shape‑selective separations of long‑chain hydrophobic compounds, providing exceptional performance for positional and geometric isomers that cannot be fully resolved with traditional C18 phases.
This column features a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), delivering enhanced hydrophobic interaction and a distinct selectivity profile that enables accurate differentiation of closely related compounds.
The 200 Å pore size makes it particularly effective for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining excellent peak shape and reproducibility.
✅ Comparable selectivity and application performance to YMC® C30 columns, making it an ideal alternative for USP L62 methods and equivalent workflows
✅ One of the most popular C30 columns for high‑resolution analytical separations
250 mm Length – Maximum Resolution & Separation Power
The 250 mm length provides the highest resolving power in the C30 analytical range, allowing separation of complex mixtures and closely related isomers with maximum clarity.
Highest peak capacity for complex samples
Superior separation of positional and geometric isomers
Improved detection of trace impurities
✅ Use when 150 mm columns cannot achieve required separation or purity
Flow Rate & System Requirements (4.6 mm ID, 3 µm)
⚠️ Compatible with standard HPLC systems, but system pressure must be monitored carefully due to column length and particle size
Typical flow rate range: ~0.8–1.5 mL/min
Recommended starting range: ~1.0–1.2 mL/min
Longer column length increases backpressure
Extra caution with viscous mobile phases
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose C30 for Hydrophobic Separations
Maximum Shape Selectivity
Resolves positional and geometric isomers
Enables separations beyond standard C18 capability
Optimized for Larger Molecules
200 Å pore improves access for complex compounds
Ideal for carotenoids, vitamins, and peptides
Reliable Alternative to Normal Phase
Avoids instability and variability of normal phase
Maintains reproducible reversed‑phase operation
Cogent C30™ Advantages
Extended C30 Ligand
Stronger hydrophobic interaction than C18
Unique selectivity for long‑chain analytes
High Purity Silica
Consistent chromatographic performance
Reliable results across runs and methods
Method Transfer Capability
Comparable to YMC® C30 column chemistry and applications
Suitable for transferring L62 methods with minimal modification
Typical Applications
Carotenoid and vitamin analysis
Isomer and positional compound separations
Peptide and large molecule separations
Hydrophobic compound profiling
High‑resolution method development
Important Usage Notes
Designed for standard analytical HPLC systems (4.6 mm ID)
Not end‑capped (intentional for enhanced selectivity)
Higher backpressure due to 3 µm particle and 250 mm length
Ideal for maximum‑resolution, shape‑selective separations