Catalog Number - 72530-05P

The 72530‑05P Cogent C30™ HPLC Column is designed for shape‑selective separation of long‑chain hydrophobic compounds, delivering improved resolution of positional and geometric isomers compared to traditional C18 phases.

This column utilizes a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and a distinct selectivity profile that enables separations not achievable with standard reversed‑phase methods.

With a 200 Å pore size, it is especially well suited for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining reliable chromatographic performance.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a dependable alternative for USP L62 methods and equivalent workflows

50 mm Length – Fast Screening & Method Development

The 50 mm length is optimized for rapid separations, making it ideal for screening and early method development.

• Short run times and fast gradients

• Efficient evaluation of selectivity for complex mixtures

• Reduced solvent consumption

✅ Use when speed and method development flexibility are priorities

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

⚠️ Compatible with standard HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Lower backpressure compared to 3 µm columns

• Standard analytical detector compatibility

• Stable flow delivery required for reproducibility

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond traditional C18 capability

Effective for Larger Molecules

• 200 Å pore improves access for complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and variability of normal phase

• Maintains consistent, reproducible operation

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent column‑to‑column performance

• Reliable chromatographic reproducibility

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• Method development and optimization

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Not end‑capped (intentional for selectivity differences)

• Lower backpressure vs smaller particle sizes

• Ideal for routine analytical and screening workflows