Catalog Number - 72530-10P-2

The 72530‑10P‑2 Cogent C30™ HPLC Column is designed for shape‑selective separation of long‑chain hydrophobic compounds, offering improved resolution for positional and geometric isomers compared to standard C18 columns.

This column features a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and a unique selectivity profile that enables separations not achievable with conventional reversed‑phase methods.

With a 200 Å pore size, it is well suited for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent chromatographic performance.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a reliable alternative for USP L62 methods and equivalent workflows

100 mm Length – Increased Resolution for LC‑MS Methods

The 100 mm length provides a strong balance between speed and resolution, making it ideal for improving separations after initial screening.

• Better separation than 50 mm columns

• Increased peak capacity for complex samples

• Improved resolution of closely related isomers

✅ Use when additional resolving power is required without moving to longer run times of 150 mm columns

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

⚠️ Requires low‑flow HPLC or LC‑MS system capability

• Typical flow rate range: ~0.2–0.3 mL/min

• Lower backpressure compared to 3 µm columns

• Requires stable, precise low‑flow pump operation

• Compatible with low‑volume LC‑MS detectors

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond C18 capability

Optimized for Larger Molecules

• 200 Å pore improves interaction with complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and reproducibility issues

• Maintains consistent, reliable operation

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic retention vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent performance across runs

• Reliable chromatographic reproducibility

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• LC‑MS method development

Important Usage Notes

• Designed for low‑flow LC‑MS systems (~0.2–0.3 mL/min)

• Not end‑capped (intentional for selectivity differences)

• Lower backpressure vs smaller particle sizes

• Ideal for routine LC‑MS and method development workflows