Catalog Number - 72530-15P

The 72530‑15P Cogent C30™ HPLC Column is designed for high‑resolution, shape‑selective separation of long‑chain hydrophobic compounds, delivering improved performance for positional and geometric isomers compared to conventional C18 phases.

This column utilizes a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and a distinct selectivity profile that enables separation of closely related structures under reversed‑phase conditions.

With a 200 Å pore size, it is particularly well suited for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent chromatographic performance and retention behavior.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a reliable alternative for USP L62 methods and equivalent analytical workflows

150 mm Length – High Resolution for Analytical Methods

The 150 mm length provides increased resolving power over shorter columns, enabling better separation of complex mixtures and difficult isomer systems.

• Higher resolution than 50 mm and 100 mm columns

• Improved separation of positional and geometric isomers

• Increased peak capacity for complex analytical samples

✅ Use when greater separation is required for accurate identification and quantitation

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

⚠️ Compatible with standard HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Lower backpressure compared to 3 µm columns

• Standard analytical detector compatibility

• Stable flow delivery recommended for reproducibility

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond traditional C18 capability

Optimized for Larger Molecules

• 200 Å pore supports complex analyte interaction

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and reproducibility issues

• Maintains consistent analytical performance

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction than C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent column‑to‑column performance

• Reliable chromatographic reproducibility

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal modification

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• Analytical method development and optimization

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Not end‑capped (intentional for enhanced selectivity)

• Lower backpressure vs smaller particle sizes

• Ideal for high‑resolution analytical workflows