Catalog Number - 72530-15P-2

The 72530‑15P‑2 Cogent C30™ HPLC Column is designed for high‑resolution, shape‑selective separation of long‑chain hydrophobic compounds, delivering improved performance for positional and geometric isomers compared to traditional C18 phases.

This column uses a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and a distinct selectivity profile that enables separation of structurally similar analytes under reversed‑phase conditions.

The 200 Å pore size enhances accessibility for larger molecules, including carotenoids, vitamins, peptides, and some proteins, making it especially useful in advanced analytical and LC‑MS workflows.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a reliable alternative for USP L62 methods and equivalent LC‑MS applications

150 mm Length – High Resolution for LC‑MS Workflows

The 150 mm length provides increased resolving power in the 2.1 mm format, enabling improved separation of closely related compounds and isomers.

• Higher resolution than 50 mm and 100 mm columns

• Improved separation of positional and geometric isomers

• Increased peak capacity for complex and trace-level samples

✅ Use when maximum LC‑MS resolution is required beyond 100 mm columns

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

⚠️ Requires low‑flow HPLC or LC‑MS system capability

• Typical flow rate range: ~0.2–0.3 mL/min

• Lower backpressure vs 3 µm columns

• Requires precise and stable low‑flow pump performance

• Compatible with low‑volume LC‑MS detector systems

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

High Shape Selectivity

• Resolves positional and geometric isomers

• Provides separation capabilities beyond C18

Optimized for Larger Molecules

• 200 Å pore supports complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and variability of normal phase

• Maintains reproducible and robust operation

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent performance across runs

• Reliable chromatographic reproducibility

Method Transfer Confidence

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• LC‑MS method development and high‑resolution workflows

Important Usage Notes

• Designed for low‑flow LC‑MS systems (~0.2–0.3 mL/min)

• Not end‑capped (intentional for enhanced selectivity)

• Lower backpressure vs smaller particle sizes

• Ideal for high‑resolution LC‑MS and method refinement workflows