Catalog Number - 75018-05P-2

The 75018‑05P‑2 Cogent HPS C18™ HPLC Column is engineered for high‑purity, low‑flow chromatographic performance, using monofunctional C18 bonding on proprietary high‑purity silica with full end‑capping.

This phase provides consistent retention, excellent peak shape, and low background interference, making it ideal for USP L1 methods and LC‑MS workflows requiring reproducibility and sensitivity.

✅ Frequently used as a cost‑effective alternative to Inertsil™ C18 and Luna™ C18 columns

50 mm Length – Fast LC‑MS & Method Development

• Designed for rapid separations and short run times

• Ideal for:

  • method development and screening

  • high‑throughput LC‑MS workflows

• Reduces:

  • solvent consumption

  • analysis time

✅ Preferred for fast method optimization and routine screening

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Optimized for low‑flow HPLC and LC‑MS systems

• Enhances mass spectrometry sensitivity via reduced solvent flow

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires HPLC/UHPLC systems capable of stable low‑flow delivery

⚠️ Ensure:

• Precise pump control

• Low‑volume detector flow cell compatibility

5 µm Particle – Reliable Performance & System Compatibility

• Provides:

  • Consistent efficiency for routine LC‑MS methods

  • Lower backpressure vs smaller particles

  • Long column lifetime

✅ Ideal for:

• LC‑MS systems without UHPLC pressure requirements

• Method transfer and reproducibility across systems

120 Å Pore Size – Enhanced Versatility

• Slightly larger than standard 100 Å phases

• Improves interaction with:

  • moderately larger molecules

  • complex analytes

✅ Provides balanced retention and selectivity across a wide compound range

Why Choose HPS C18™ Columns

High‑Purity Silica Platform

• Reduces:

  • metal content

  • secondary interactions

• Improves:

  • peak symmetry

  • analytical reproducibility

Monofunctional C18 Bonding

• Provides:

  • controlled, predictable surface chemistry

  • improved method consistency

✅ Ideal for method development and transfer

LC‑MS Performance Advantage

• Clean baseline and low bleed

• Suitable for high‑sensitivity detection workflows

✅ Preferred for trace‑level LC‑MS analysis

Cost‑Performance Advantage

• Comparable to:

  • Inertsil™ C18

  • Luna™ C18

✅ Delivers premium‑level performance at competitive cost

Application Performance Advantages

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Provides:

  • Longer retention vs C8 phases

  • Reliable performance across LC‑MS workflows

✅ Ideal for general‑purpose reversed‑phase LC‑MS analysis

System & Sample Considerations

• Designed for low‑flow LC‑MS systems (2.1 mm ID)

• Suitable for routine and high‑sensitivity workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column clogging

• Maintain long‑term LC‑MS performance

Typical Applications

• LC‑MS and LC‑MS/MS workflows

• USP L1 analytical methods

• Pharmaceutical QC and validation

• Method development and screening

• Hydrophobic compound separations

• Trace‑level analytical workflows

Important Usage Notes

• Designed for low‑flow LC‑MS systems (~0.2–0.3 mL/min)

• Requires stable pump control for best performance

• Ideal for fast analysis and high‑throughput workflows

• Monitor system pressure relative to instrument capabilities