Catalog Number - 75018-25P

The 75018‑25P Cogent HPS C18™ HPLC Column is engineered for maximum‑resolution analytical separations, delivering consistent retention, excellent peak symmetry, and reproducible selectivity.

Built with monofunctional C18 bonding on proprietary high‑purity silica and fully double end‑capped, this column reduces secondary interactions and ensures reliable performance for USP L1 methods and demanding analytical applications.

✅ One of the most popular HPS™ columns for high‑resolution workflows ✅ Frequently used as a cost‑effective alternative to Inertsil™ C18 and Luna™ C18 columns

250 mm Length – Maximum Resolution & Separation Power

• Highest resolving power in the HPS analytical column range

• Maximizes:

  • Peak capacity

  • Separation of closely eluting analytes

  • Impurity and trace‑level detection

• Provides:

  • Extended retention time window

  • Enhanced separation of complex mixtures

✅ Selected when shorter columns (50 mm / 100 mm / 150 mm) cannot achieve required resolution

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method validation and regulatory workflows

  • routine QC analysis

  • complex sample separations

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Provides robust, reproducible analytical performance

5 µm Particle – Proven Analytical Performance

• Provides:

  • Reliable efficiency for long analytical runs

  • Lower backpressure vs smaller particles

  • Long column lifetime

✅ Ideal for:

• Routine and regulated workflows

• Method transfer across laboratories and systems

120 Å Pore Size – Enhanced Versatility & Selectivity

• Larger than traditional 100 Å C18 phases

• Improves interaction with:

  • moderately larger analytes

  • complex molecular structures

✅ Provides broader application range and improved chromatographic flexibility

Why Choose HPS C18™ Columns

High‑Purity Silica Platform

• Reduces:

  • metal contamination

  • secondary interactions

• Improves:

  • peak symmetry

  • reproducibility and accuracy

Monofunctional C18 Bonding

• Provides:

  • controlled surface chemistry

  • predictable selectivity

✅ Ideal for method development and validation workflows

Cost‑Performance Advantage

• Comparable to:

  • Inertsil™ C18

  • Luna™ C18

✅ Delivers premium‑level performance at competitive cost

Application Performance Advantages

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Provides:

  • Strong retention vs C8 columns

  • High resolution for complex mixtures

✅ Ideal for advanced analytical HPLC workflows and impurity profiling

System & Sample Considerations

• Compatible with standard HPLC systems

• Suitable for long‑run analytical workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain maximum performance and longevity

Typical Applications

• USP L1 analytical methods

• Pharmaceutical QC and validation

• Impurity profiling and trace analysis

• Complex mixture separations

• Method development and optimization

• Long‑chain fatty acid analysis

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Suitable for USP L1 methods and advanced method development

• Ideal for maximum‑resolution analytical workflows

• Monitor system pressure based on instrument capabilities