Prep HPLC Column, HPS C18, 5um, 120A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 75018‑P100 Cogent HPS C18™ Preparative Column is designed for increased‑resolution purification and scalable reversed‑phase workflows, providing consistent retention, strong peak symmetry, and reliable selectivity.
Manufactured using high‑purity silica with monofunctional C18 bonding and full double end‑capping, this phase minimizes secondary interactions and ensures stable chromatographic performance during scale‑up from analytical and semi‑prep columns.
It is particularly well suited for purification of hydrophobic and neutral compounds, including long‑chain fatty acids, where both resolution and throughput are important.
100 mm Length – Increased Resolution for Preparative Work
The 100 mm length provides a balance between throughput and resolution, offering improved separation compared to 50 mm columns while maintaining efficient cycle times.
Better separation of closely eluting compounds
Improved fraction purity vs short columns
Efficient balance of run time and resolution
✅ Use when 50 mm does not achieve sufficient separation, but maximum resolution is not yet required
Flow Rate & System Requirements (21.2 mm ID, 5 µm)
⚠️ Requires a preparative HPLC system capable of high flow rates and large injection volumes
Typical flow rate range: ~15–40 mL/min
High sample loading capability required
Preparative detector flow cell recommended
Stable high‑flow pumping essential for reproducibility
✅ Sample preparation requirement: use appropriate filtration (0.2 µm or 0.45 µm) to prevent clogging and protect column performance
Why Choose HPS C18 for Preparative Work
Balanced Resolution & Throughput
Longer than 50 mm for improved separation
Shorter than 150–250 mm for faster cycle times
Scalable Method Transfer
Maintains selectivity across 4.6 mm → 10 mm → 21.2 mm
Enables direct scale‑up without method redevelopment
High‑Purity Silica Performance
Reduces variability and secondary interactions
Improves fraction consistency and recovery
Cogent HPS C18™ Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak shape, especially for difficult analytes
Monofunctional Bonding
Predictable retention and selectivity
Consistent performance across workflows
Preparative Durability
Lower backpressure vs smaller particles
Designed for repeated high‑load purification cycles
Typical Applications
Preparative purification
Method scale‑up from analytical and semi‑prep columns
Hydrophobic and neutral compound isolation
Fraction collection workflows
Pharmaceutical process development
Important Usage Notes
Requires preparative HPLC system (21.2 mm ID compatibility)
Not suitable for standard analytical systems
Designed for high‑flow purification workflows
👉 Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable