Prep HPLC Column, HPS C18, 5um, 120A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.

Additional Info:

The 75018‑P250 Cogent HPS C18™ Preparative Column is designed for maximum‑resolution purification and production‑scale separations, delivering strong retention, excellent peak symmetry, and highly reproducible results.

Manufactured with high‑purity silica, monofunctional C18 bonding, and full double end‑capping, this stationary phase minimizes secondary interactions and ensures consistent chromatographic performance during full scale‑up from analytical and semi‑preparative methods.

This column is particularly effective for purification of hydrophobic and neutral compounds, including long‑chain fatty acids, where the highest level of separation and compound purity is required.

250 mm Length – Maximum Resolution & Purification Power

The 250 mm length provides the highest resolving power in the preparative HPS range, enabling separation of closely related impurities and trace components.

  • Maximum separation for complex mixtures

  • Highest peak capacity for difficult samples

  • Superior fraction purity and isolation accuracy

✅ Use when 150 mm columns do not achieve required purity or separation performance

Flow Rate & System Requirements (21.2 mm ID, 5 µm)

⚠️ Requires a preparative HPLC system designed for high flow rates and large sample loading

  • Typical flow rate range: ~15–40 mL/min

  • High injection volumes required for production work

  • Preparative detector flow cell recommended

  • System must deliver stable, high‑flow performance

✅ Sample preparation requirement: use appropriate filtration (0.2 µm or 0.45 µm) to prevent clogging and protect column lifetime

Why Choose HPS C18 for Preparative Work

Maximum Resolution for Production Workflows

  • Enables purification of closely related compounds and impurities

  • Ideal for demanding purification applications

Seamless Method Scaling

  • Maintains selectivity across 4.6 mm → 10 mm → 21.2 mm columns

  • Eliminates need for redevelopment during scale‑up

High‑Purity Silica Platform

  • Reduces variability and secondary interactions

  • Improves reproducibility and fraction consistency

Cogent HPS C18™ Advantages

Fully End‑Capped Surface

  • Minimizes silanol activity

  • Improves peak shape for difficult analytes

Monofunctional Bonding

  • Predictable, stable selectivity

  • Consistent chromatographic behavior across workflows

Preparative Durability

  • Lower backpressure than smaller particles

  • Designed for repeated, high‑load purification cycles

Typical Applications

  • Preparative purification at scale

  • Process and production chromatography

  • Hydrophobic and neutral compound isolation

  • Impurity profiling and fraction collection

  • Pharmaceutical and chemical manufacturing workflows

Important Usage Notes

  • Requires preparative HPLC system (21.2 mm ID compatibility)

  • Not suitable for standard analytical systems

  • Designed for high‑flow, production‑scale purification workflows

👉 Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

  • Columns are manufactured specifically to order

  • Orders cannot be canceled once placed

  • Custom columns are non‑returnable

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