Semi Prep HPLC Column, HPS C18, 5um, 120A. 10mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 75018‑SP100 Cogent HPS C18™ Semi‑Preparative Column is designed for higher‑resolution purification and scalable method development, delivering consistent retention, strong peak shape, and reliable selectivity.
Manufactured with high‑purity silica, monofunctional C18 bonding, and full double end‑capping, this column minimizes secondary interactions and provides stable performance across a wide range of reversed‑phase applications. It is especially effective when scaling methods from analytical HPLC to semi‑prep purification.
100 mm Length – Improved Separation Before Scale‑Up
At 100 mm, this column provides more resolving power than short (50 mm) screening columns, allowing better separation of closely eluting compounds and improved fraction purity during purification.
Use this length when:
50 mm does not provide adequate separation
Higher purity fractions are required before scale‑up
10 mm ID – Semi‑Preparative Operation
The 10 mm internal diameter supports larger injection volumes and higher sample loading, making it suitable for purification workflows rather than analytical runs.
Typical operation is in the range of ~3–6 mL/min, depending on the system and method. This format requires a dedicated semi‑prep HPLC system capable of stable higher flow rates and larger volume handling.
Particle Size and Pore Structure
This column uses a 5 µm particle, which provides a practical balance between efficiency and backpressure, making it reliable for repeated injections and routine purification work.
The 120 Å pore size offers broader compound compatibility compared to 100 Å phases, improving interaction with moderately larger or more complex analytes while maintaining strong retention of hydrophobic compounds.
Application Use
This column is well suited for:
Semi‑preparative purification
Analytical‑to‑prep method scaling
Hydrophobic and neutral compound isolation
Fraction collection workflows
It is commonly used in process development where consistent selectivity must be preserved during scale‑up.
Important Notes
This column requires a semi‑preparative HPLC system and is not intended for standard analytical instruments.
⚠️ Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable