Semi Prep HPLC Column, HPS C18, 5um, 120A. 10mm ID x 150mm Length. Cogent Brand. 1 EA.
Additional Info:
The 75018‑SP150 Cogent HPS C18™ Semi‑Preparative Column is designed for high‑resolution purification and scalable reversed‑phase workflows, delivering strong retention, excellent peak shape, and consistent selectivity.
Manufactured with high‑purity silica, monofunctional C18 bonding, and full double end‑capping, this stationary phase minimizes secondary interactions and provides reliable chromatographic performance during analytical‑to‑semi‑prep method transfer.
This makes HPS C18 an excellent choice for purification of hydrophobic and neutral compounds, including long‑chain fatty acids, where consistent retention and reproducibility are critical.
150 mm Length – Maximum Resolution for Semi‑Prep Work
The 150 mm length provides enhanced resolving power compared to 50 mm and 100 mm columns, allowing better separation of closely related compounds and improved purity of collected fractions.
Higher resolution for difficult separations
Improved fraction purity and recovery
Increased peak capacity for complex mixtures
✅ Use when maximum separation is required before scaling to larger preparative columns
Flow Rate & System Requirements (10 mm ID, 5 µm)
⚠️ Requires a semi‑preparative HPLC system designed for higher flow rates and larger sample loading
Typical flow rate range: ~3–6 mL/min
Higher injection volume capability required
Optimized detector flow cell recommended for larger flows
Stable pumping performance at elevated flow rates
✅ Sample preparation requirement: use appropriate filtration (0.2 µm or 0.45 µm) to protect column performance and prevent clogging
Why Choose HPS C18 for Semi‑Prep
Scalable Method Transfer
Maintains selectivity from analytical (4.6 mm) to semi‑prep (10 mm)
Eliminates need to redevelop methods during scale‑up
High‑Purity Silica Performance
Reduces secondary interactions and variability
Improves peak symmetry and reproducibility
Efficient Purification Workflow
Reliable retention for hydrophobic compounds
Supports consistent fraction collection
Cogent HPS C18™ Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improved peak shape for difficult compounds
Monofunctional Bonding
Predictable selectivity across methods
Consistent chromatographic behavior
Balanced Performance
Lower backpressure vs smaller particles
Durable for repeated purification cycles
Typical Applications
Semi‑preparative purification
Method scaling from analytical HPLC
Hydrophobic and neutral compound isolation
Fraction collection and compound recovery
Pharmaceutical process development
Important Usage Notes
Requires semi‑preparative HPLC system (10 mm ID compatibility)
Not suitable for standard analytical systems
Designed for high‑flow purification workflow
👉 Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable