Semi Prep HPLC Column, HPS C18, 5um, 120A. 10mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
The 75018‑SP250 Cogent HPS C18™ Semi‑Preparative Column is designed for maximum‑resolution purification and advanced method scaling, delivering strong retention, excellent peak symmetry, and highly reproducible separations.
Manufactured with high‑purity silica, monofunctional C18 bonding, and full double end‑capping, this phase minimizes secondary interactions and provides consistent chromatographic behavior during scale‑up from analytical to semi‑prep workflows.
This column is especially effective for purification of hydrophobic and neutral compounds, including long‑chain fatty acids, where maximum separation and fraction purity are required.
250 mm Length – Maximum Resolution & Purity
At 250 mm, this column provides the highest resolving power in the semi‑prep HPS range, enabling separation of closely related compounds and trace impurities.
Maximum separation for complex mixtures
Highest peak capacity for difficult samples
Improved fraction purity and isolation accuracy
✅ Use when 150 mm columns cannot achieve required purity or resolution
Flow Rate & System Requirements (10 mm ID, 5 µm)
⚠️ Requires a semi‑preparative HPLC system capable of higher flow rates and sample loading
Typical flow rate range: ~3–6 mL/min
Higher injection volumes required for purification
Detector must support larger flow cell volume
System must provide stable high‑flow operation
✅ Sample preparation requirement: use appropriate filtration (0.2 µm or 0.45 µm) to protect column performance and prevent clogging
Why Choose HPS C18 for Semi‑Prep
Maximum Resolution for Scale‑Up
Provides highest separation power before moving to prep or purification columns
Ideal for impurity isolation and difficult separations
Scalable Method Transfer
Matches analytical HPS selectivity (4.6 mm → 10 mm)
Eliminates need to redevelop methods during scale‑up
High‑Purity Silica Platform
Reduces secondary interactions
Improves reproducibility and fraction recovery
Cogent HPS C18™ Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry for difficult analytes
Monofunctional Bonding
Predictable and consistent selectivity
Stable retention across workflows
Durable Semi‑Prep Performance
Lower backpressure than smaller particles
Designed for repeated high‑load injections
Typical Applications
Semi‑preparative purification
Method scaling and process development
Hydrophobic and neutral compound isolation
Impurity profiling and fraction collection
Pharmaceutical and analytical purification workflows
Important Usage Notes
Requires semi‑preparative HPLC system (10 mm ID compatibility)
Not suitable for standard analytical systems
Designed for high‑flow purification and scale‑up workflows
👉 Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable