Catalog Number - 75040-10P-2

Cogent HPS Amino™ HPLC Columns are designed for the analysis of highly polar compounds using Normal Phase and HILIC chromatography modes. Manufactured using tri-functional bonding of a three-carbon silane with a terminal amino functional group bonded to high-purity silica, this stationary phase is not end capped and provides the selectivity required for challenging separations of carbohydrates, sugars, organic acids, and other polar analytes. HPS Amino™ is widely used as an alternative for USP L8 methods and for new method development requiring amino phase selectivity.

75040-10P-2 is packed with 5 µm HPS Amino™ particles in a 2.1 mm ID x 100 mm analytical format. The narrow-bore design reduces solvent consumption while supporting enhanced sensitivity in LC-MS applications. The 100 mm length provides an excellent balance of chromatographic resolution and analysis speed, while the 5 µm particle size delivers reliable efficiency with lower operating backpressure than smaller particles.

The HPS Amino™ stationary phase is particularly effective for negatively charged polar compounds, including sugars, carbohydrates, and compounds containing carboxylic acid functional groups. Commonly used in both Normal Phase and HILIC chromatography, this column provides excellent retention and selectivity for analytes that can be difficult to retain on traditional reversed-phase columns. The 2.1 mm ID format is especially popular for LC-MS workflows where reduced flow rates can improve sensitivity and lower solvent consumption.

Key Benefits

• HPS Amino™ stationary phase

• USP L8 equivalent chemistry

• Tri-functional amino bonding

• High-purity silica support

• 5 µm particle size

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Reduced solvent consumption

• Excellent for sugars and organic acids

• Highly suitable for LC-MS applications

Typical Applications

• USP L8 methods

• HILIC chromatography

• Normal Phase HPLC

• LC-MS and LC-MS/MS

• Sugar analysis

• Carbohydrate analysis

• Organic acid analysis

• Polar compound separations

• Method development

• Quality control testing

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: HPS Amino™

• USP Classification: L8

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L8 equivalent chemistry

• Not end capped

• Designed for polar and negatively charged compounds

• Excellent for sugars and compounds containing carboxylic acid groups

• Particularly well suited for LC-MS applications

• 2.1 mm ID columns require an HPLC system capable of reliable low-flow operation

• Low-volume detector flow cells are recommended for optimal performance

• Frequently compared with Inertsil™ Amino and Luna™ Amino columns

• 100 mm length provides an excellent balance of speed and resolution

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