Catalog Number - 75040-25P

Cogent HPS Amino™ HPLC Columns are designed for the analysis of highly polar compounds using Normal Phase and HILIC chromatography modes. Manufactured using tri-functional bonding of a three-carbon silane with a terminal amino functional group bonded to high-purity silica, this stationary phase is not end capped and provides the selectivity required for challenging separations of carbohydrates, sugars, organic acids, and other polar analytes. HPS Amino™ is widely used as an alternative for USP L8 methods and for new method development requiring amino phase selectivity.

75040-25P is packed with 5 µm HPS Amino™ particles in a 4.6 mm ID x 250 mm analytical format. The 250 mm length provides maximum retention, chromatographic resolution, and peak capacity for demanding analytical separations. The 5 µm particle size delivers reliable efficiency with lower operating backpressure than smaller particle columns, making it suitable for conventional HPLC systems while offering the resolving power needed for complex sample matrices.

The HPS Amino™ stationary phase is particularly effective for negatively charged polar compounds, including sugars, carbohydrates, and compounds containing carboxylic acid functional groups. Commonly used in both Normal Phase and HILIC chromatography, this column provides excellent retention and selectivity for analytes that may be difficult to retain using traditional reversed-phase methods. The extended 250 mm length is ideal when maximum separation performance is required for complex or closely related compounds.

Key Benefits

• HPS Amino™ stationary phase

• USP L8 equivalent chemistry

• Tri-functional amino bonding

• High-purity silica support

• 5 µm particle size

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Lower operating backpressure

• Excellent for sugars and organic acids

• Maximum resolution and peak capacity

Typical Applications

• USP L8 methods

• HILIC chromatography

• Normal Phase HPLC

• Sugar analysis

• Carbohydrate analysis

• Organic acid analysis

• Polar compound separations

• Food and beverage testing

• Method development

• Complex analytical separations

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: HPS Amino™

• USP Classification: L8

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L8 equivalent chemistry

• Not end capped

• Designed for polar and negatively charged compounds

• Excellent for sugars and compounds containing carboxylic acid groups

• Compatible with standard analytical HPLC systems

• Frequently compared with Inertsil™ Amino and Luna™ Amino columns

• Lower backpressure than smaller particle columns

• 250 mm length provides maximum retention, resolution, and peak capacity

• Ideal for complex separations and challenging analytical methods

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