Catalog Number - 82061-05P

Cogent NP Silica™ HPLC Columns are designed for the separation of hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 100Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for many traditional Normal Phase and HILIC applications. NP Silica™ is widely used as an alternative for USP L3 methods and for developing new analytical, preparative, and purification methods requiring the unique retention characteristics of unbonded silica.

82061-05P is packed with 10 µm NP Silica™ particles in a 4.6 mm ID x 50 mm analytical format. The larger 10 µm particle size generates significantly lower backpressure than smaller particle columns, allowing the use of higher flow rates while maintaining dependable chromatographic performance. The short 50 mm length is ideal for rapid method development, fast screening studies, and solvent-efficient scouting applications.

NP Silica™ 10 µm columns are very often used as scouting columns for preparative chromatography, purification methods, and process development workflows. The 50 mm format allows rapid evaluation of retention, selectivity, and mobile phase conditions before scaling methods to larger preparative and purification columns. Because the stationary phase chemistry remains consistent, methods developed on this column can often be transferred directly to larger ID preparative and purification columns with predictable chromatographic behavior, reducing development time and cost.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 10 µm particle size

• Lower operating backpressure

• Suitable for higher flow rates

• Ideal for preparative method scouting

• Suitable for HILIC and Normal Phase chromatography

Typical Applications

• USP L3 methods

• HILIC chromatography

• Normal Phase HPLC

• Preparative method development

• Purification method scouting

• Process scale-up studies

• Polar compound analysis

• Hydrophilic compound analysis

• Method transfer to preparative columns

• High-throughput screening

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Recommended Flow Rate: 1.0–3.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent chemistry

• Unmodified silica stationary phase

• Low metal content, high-purity silica

• Designed for hydrophilic and polar compounds

• Produces lower operating backpressure than smaller particle columns

• Supports higher flow rates than 3 µm and 5 µm particle columns

• Frequently used as a scouting column for preparative methods

• Excellent for purification method development and process scale-up

• Methods can often be transferred directly to larger preparative and purification columns with the same stationary phase

• Compatible with standard analytical HPLC systems

• 50 mm length minimizes solvent consumption and analysis time

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