Catalog Number - 82061-10P

Cogent NP Silica™ HPLC Columns are designed for the separation of hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 100Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for many traditional Normal Phase and HILIC applications. NP Silica™ is widely used as an alternative for USP L3 methods and for developing new analytical, preparative, and purification methods requiring the unique retention characteristics of unbonded silica.

82061-10P is packed with 10 µm NP Silica™ particles in a 4.6 mm ID x 100 mm analytical format. The larger 10 µm particle size generates substantially lower backpressure than smaller particle columns, allowing operation at higher flow rates while maintaining dependable chromatographic performance. The 100 mm length provides an excellent balance of retention, resolution, and analysis time, making it well suited for both method development and routine chromatography applications.

NP Silica™ 10 µm columns are very often used as scouting columns for preparative chromatography, purification methods, and process scale-up projects. The 100 mm format provides increased retention and resolution compared to shorter scouting columns while still allowing rapid evaluation of selectivity and mobile phase conditions. Because the stationary phase chemistry remains consistent, methods developed on this column can often be scaled directly to larger preparative and purification columns with predictable chromatographic behavior, helping reduce development time and facilitate efficient scale-up.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 10 µm particle size

• Lower operating backpressure

• Suitable for higher flow rates

• Ideal for preparative method scouting

• Suitable for HILIC and Normal Phase chromatography

Typical Applications

• USP L3 methods

• HILIC chromatography

• Normal Phase HPLC

• Preparative method development

• Purification method scouting

• Process scale-up studies

• Polar compound analysis

• Hydrophilic compound analysis

• Method transfer to preparative columns

• High-throughput screening

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Recommended Flow Rate: 1.0–3.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent chemistry

• Unmodified silica stationary phase

• Low metal content, high-purity silica

• Designed for hydrophilic and polar compounds

• Produces lower operating backpressure than smaller particle columns

• Supports higher flow rates than 3 µm and 5 µm particle columns

• Frequently used as a scouting column for preparative methods

• Excellent for purification method development and process scale-up

• Methods can often be transferred directly to larger preparative and purification columns with the same stationary phase

• Compatible with standard analytical HPLC systems

• 100 mm length provides an excellent balance of speed and resolution

  ---