Catalog Number - 82061-25P

Cogent NP Silica™ HPLC Columns are manufactured using ultra-high purity, low metal content, 100Å silica that remains completely unmodified, providing the native silica selectivity required for Normal Phase and HILIC chromatography. This highly regarded stationary phase is widely used as an alternative for methods requiring USP L3 equivalent chemistry and is particularly effective for the separation of hydrophilic and polar compounds that may not be adequately retained using reversed-phase methods.

82061-25P is packed with 10 µm NP Silica™ particles in a 4.6 mm ID x 250 mm analytical format. The larger particle size generates significantly lower operating backpressure than smaller particle columns, allowing higher flow rates while maintaining reliable chromatographic performance. The 250 mm column length provides increased retention, peak capacity, and chromatographic resolution, making it an excellent choice for demanding analytical separations and complex sample matrices.

The 10 µm particle size makes this column especially valuable for preparative method development, purification method scouting, and process scale-up studies. Because the stationary phase chemistry remains consistent across analytical, preparative, and purification column formats, methods developed on this column can often be scaled directly to larger internal diameter columns with predictable chromatographic performance. This approach helps reduce development time and simplifies transfer to preparative and purification workflows.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 100Å pore size

• 10 µm particle size

• Lower operating backpressure

• Ideal for preparative method scouting

• Suitable for HILIC and Normal Phase chromatography

Typical Applications

• Hydrophilic compound analysis

• Polar compound separations

• HILIC chromatography

• Normal Phase chromatography

• USP L3 method replacement

• Pharmaceutical analysis

• Method development

• Preparative method scouting

• Purification method development

• Process scale-up studies

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 100Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0–3.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• Designed for hydrophilic and polar compound separations

• Lower operating pressure than 3 µm and 5 µm particle columns

• Supports higher operating flow rates

• Compatible with standard analytical HPLC systems

• Frequently used for preparative chromatography scouting

• Excellent for purification method development and process scale-up

• Methods can often be transferred directly to larger preparative and purification columns using the same stationary phase

• 250 mm length provides maximum retention and chromatographic resolution for demanding separations

• Ideal for method development requiring enhanced peak capacity and separation efficiency