Prep HPLC Column, NP Silica, Wide Pore, 10um, 300A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Silica™ Wide Pore Preparative HPLC Columns are trusted by leading laboratories and pilot plants worldwide for compound purification, method scale-up, and process chromatography applications. Manufactured from ultra-high purity, low metal content, 300Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for the purification of proteins, peptides, biomolecules, lipids, and other large hydrophilic or polar compounds. The wide pore structure improves accessibility for larger analytes and supports both HILIC and Normal Phase chromatography.
82061-P21-1003M is packed with 10 µm NP Silica™ Wide Pore particles in a 21.2 mm ID x 100 mm preparative column format. The larger particle size allows operation at higher flow rates while generating lower backpressure than smaller particle columns. The 21.2 mm internal diameter provides substantial sample loading capacity for preparative separations, compound isolation, and purification workflows, while the 100 mm length offers an excellent balance between throughput and chromatographic performance.
For many years, 10 µm NP Silica™ Wide Pore columns have been used in pharmaceutical, biotechnology, cannabis, chemical, and forensic laboratories around the world for preparative HPLC and purification applications. The 300Å pore structure is particularly beneficial for large molecules such as proteins and peptides, while the stationary phase chemistry scales directly from analytical columns to preparative and purification formats. Methods developed on analytical NP Silica™ Wide Pore columns can often be transferred directly to larger ID columns with predictable chromatographic behavior, helping streamline scale-up and production workflows.
Key Benefits
NP Silica™ Wide Pore stationary phase
USP L3 equivalent chemistry
Ultra-high purity silica
Low metal content silica
Unmodified silica surface
300Å wide pore design
10 µm particle size
Lower operating backpressure
High sample loading capacity
Ideal for preparative HPLC and purification
Typical Applications
Preparative HPLC
Protein purification
Peptide purification
Biomolecule isolation
Large molecule separations
HILIC chromatography
Normal Phase chromatography
Pharmaceutical purification
Cannabis purification
Process-scale chromatography
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: NP Silica™ Wide Pore
USP Classification: L3
Chromatography Modes: HILIC and Normal Phase
Particle Size: 10 µm
Pore Size: 300Å
Column Dimensions: 21.2 mm ID x 100 mm
Recommended Flow Rate: 15–40 mL/min
Sample Loading Capacity: Preparative Scale
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Suitable for HILIC and Normal Phase chromatography
USP L3 equivalent stationary phase
Unmodified silica surface chemistry
Manufactured from ultra-high purity, low metal content silica
300Å wide pore structure provides improved accessibility for proteins, peptides, and other large molecules
Designed for purification of large hydrophilic, polar, and charged compounds
Lower operating pressure than smaller particle preparative columns
High loading capacity for compound isolation and purification
Frequently used in pharmaceutical, biotechnology, cannabis, chemical, and forensic laboratories
Proven preparative HPLC technology used globally for many years
Directly scalable from analytical NP Silica™ Wide Pore column methods
Excellent for method scale-up, purification development, and process chromatography
Requires a preparative HPLC system capable of supporting higher flow rates and larger column dimensions
100 mm length provides an effective balance of purification throughput and chromatographic performance
Special Order Item Information
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