Catalog Number - 82061-P50-2503M

Cogent NP Silica™ Wide Pore Process HPLC Columns are trusted by leading laboratories, pilot plants, and production facilities worldwide for large-scale purification, process chromatography, and manufacturing applications. Manufactured from ultra-high purity, low metal content, 300Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for the purification of proteins, peptides, biomolecules, lipids, and other large hydrophilic or polar compounds. The wide pore structure improves analyte accessibility and is especially beneficial for higher molecular weight compounds in both HILIC and Normal Phase chromatography.

82061-P50-2503M is packed with 10 µm NP Silica™ Wide Pore particles in a 50 mm ID x 250 mm process column format. The larger particle size enables operation at high flow rates while generating substantially lower backpressure than smaller particle columns. The 50 mm internal diameter provides exceptional sample loading capacity, while the 250 mm column length maximizes retention, peak capacity, and chromatographic resolution for demanding process-scale purification, production chromatography, and manufacturing applications.

The 10 µm particle design has made this column a proven choice for preparative HPLC, process development, and production-scale chromatography worldwide. For many years, Cogent NP Silica™ Wide Pore columns have been used in the purification and manufacture of pharmaceuticals, specialty chemicals, food additives, nutraceuticals, natural products, proteins, peptides, and other high-value compounds. The 300Å pore structure provides improved accessibility for large molecules, while the stationary phase chemistry remains directly scalable from analytical and preparative formats to process-scale production columns. This enables reliable method transfer and predictable scale-up from laboratory development to pilot plant operations and commercial manufacturing.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 300Å wide pore design

• 10 µm particle size

• Lower operating backpressure

• Exceptional sample loading capacity

• Proven for process chromatography and production purification

Typical Applications

• Process HPLC

• Production chromatography

• Protein purification

• Peptide purification

• Biomolecule purification

• Large molecule separations

• HILIC chromatography

• Normal Phase chromatography

• Pharmaceutical manufacturing

• Chemical and food additive purification

Specifications

• Product Type: Process HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 300Å

• Column Dimensions: 50 mm ID x 250 mm

• Recommended Flow Rate: 80–200 mL/min

• Sample Loading Capacity: Process Scale

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• 300Å wide pore structure provides improved accessibility for proteins, peptides, and other large molecules

• Designed for purification of large hydrophilic, polar, and charged compounds

• Lower operating pressure than smaller particle process columns

• Exceptional loading capacity for production-scale purification

• Frequently used in pharmaceutical, biotechnology, chemical, food additive, nutraceutical, cannabis, and forensic applications

• Proven process chromatography technology trusted worldwide for many years

• Directly scalable from analytical and preparative NP Silica™ Wide Pore column methods

• Excellent for process development, manufacturing support, and commercial purification

• Requires a process HPLC system capable of supporting flow rates of approximately 80–200 mL/min and large-diameter columns

• 250 mm length provides maximum retention, peak capacity, and chromatographic resolution for demanding purification applications

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.