Catalog Number - 82061-SP250-3M

Cogent NP Silica™ Wide Pore Semi-Preparative HPLC Columns are trusted by laboratories worldwide for purification, method scale-up, and compound isolation applications. Manufactured from ultra-high purity, low metal content, 300Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for the separation and purification of proteins, peptides, biomolecules, and other large hydrophilic or polar compounds. Suitable for both HILIC and Normal Phase chromatography, the wide pore structure improves analyte accessibility and chromatographic performance for larger molecular weight compounds.

82061-SP250-3M is packed with 10 µm NP Silica™ Wide Pore particles in a 10 mm ID x 250 mm semi-preparative column format. The larger particle size enables operation at higher flow rates while generating lower backpressure than smaller particle columns. The 10 mm internal diameter provides significantly greater loading capacity than analytical columns, while the 250 mm length maximizes retention, peak capacity, and chromatographic resolution for demanding purification workflows involving larger molecules.

The 10 µm particle design makes this column particularly valuable for preparative method scouting, purification method development, and scale-up studies. The 300Å pore structure provides improved access for proteins, peptides, and other large molecules, while the stationary phase chemistry remains consistent across analytical, semi-preparative, preparative, and process-scale formats. Methods developed on analytical NP Silica™ Wide Pore columns can often be transferred directly to larger ID columns with predictable chromatographic behavior, helping streamline purification workflows, reduce development time, and simplify scale-up to preparative and process columns.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 300Å wide pore design

• 10 µm particle size

• Lower operating backpressure

• Increased loading capacity

• Ideal for semi-preparative purification

Typical Applications

• Semi-preparative HPLC

• Protein purification

• Peptide purification

• Biomolecule isolation

• Large molecule separations

• HILIC chromatography

• Normal Phase chromatography

• Pharmaceutical purification

• Method development

• Scale-up studies

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 300Å

• Column Dimensions: 10 mm ID x 250 mm

• Recommended Flow Rate: 8–15 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• 300Å wide pore structure provides improved accessibility for proteins, peptides, and other large molecules

• Lower operating pressure than smaller particle columns

• Increased loading capacity compared to analytical columns

• Frequently used for purification method development and semi-preparative separations

• Excellent for preparative method scouting and scale-up studies

• Methods can often be transferred directly to larger preparative and process-scale NP Silica™ Wide Pore columns

• Requires a semi-preparative HPLC system capable of supporting flow rates of approximately 8–15 mL/min

• 250 mm length provides maximum retention, peak capacity, and chromatographic resolution for demanding purification applications

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

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