Catalog Number - 82161-05P-2

Cogent NP Silica™ UHPLC Columns are designed for high-efficiency analytical separations using modern UHPLC and UPLC-type systems. Manufactured from ultra-high purity, low metal content, 100Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for HILIC and Normal Phase chromatography. Equivalent to USP L3 silica chemistry, it is an excellent choice for developing new UHPLC methods and for transferring or optimizing established USP methods that specify L3 stationary phases.

82161-05P-2 is packed with 1.8 µm NP Silica™ particles in a 2.1 mm ID x 50 mm analytical UHPLC column format. The small particle size produces exceptionally high efficiency, narrow peak widths, and increased peak capacity compared to larger particle columns. The 2.1 mm ID format significantly reduces solvent consumption while maintaining chromatographic performance, making it an ideal choice for UHPLC, LC-MS compatible workflows, and high-throughput analytical laboratories. Because of the low flow rates required, the UHPLC system must be capable of reliable low-flow operation.

This column is especially well suited for HILIC separations of polar and hydrophilic compounds. The unmodified silica surface provides strong retention of polar analytes, making it an excellent choice for the analysis of pharmaceuticals, metabolites, organic acids, sugars, amino acids, and other compounds that can be difficult to retain using traditional reversed-phase methods. The combination of ultra-high purity silica and 1.8 µm particle technology provides outstanding resolution, rapid analysis times, and increased throughput while reducing solvent consumption and laboratory operating costs.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 100Å pore size

• 1.8 µm particle size

• High efficiency separations

• Increased peak capacity

• Excellent for HILIC separations of polar compounds

Typical Applications

• UHPLC analysis

• UPLC-type systems

• HILIC chromatography

• Normal Phase chromatography

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• LC-MS compatible methods

• Method development

• High-throughput analysis

Specifications

• Product Type: UHPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 1.8 µm

• Pore Size: 100Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed exclusively for UHPLC and UPLC-type systems

• UPLC is a trademarked brand designation of Waters Corporation

• Suitable for HILIC and Normal Phase chromatography

• Excellent for HILIC retention of polar and hydrophilic compounds

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• Provides significantly higher efficiency than larger particle columns

• Ideal for reducing run times while maintaining or improving resolution

• Excellent for method development and method optimization

• Reduced solvent consumption and increased sample throughput

• The 2.1 mm ID format is well suited for LC-MS applications

• Requires a UHPLC system capable of reliable low-flow operation and elevated pressures generated by 1.8 µm particles

• Samples should be filtered using a 0.2 µm syringe filter prior to injection

• 50 mm length is optimized for rapid analysis and high-throughput workflows

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