Catalog Number - 82161-15P-2

Cogent NP Silica™ UHPLC Columns are designed for high-efficiency analytical separations using modern UHPLC and UPLC-type systems. Manufactured from ultra-high purity, low metal content, 100Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for HILIC and Normal Phase chromatography. Equivalent to USP L3 silica chemistry, it is an excellent choice for developing new UHPLC methods and for transferring or optimizing established USP methods that specify L3 stationary phases.

82161-15P-2 is packed with 1.8 µm NP Silica™ particles in a 2.1 mm ID x 150 mm analytical UHPLC column format. The small particle size produces exceptionally high efficiency, narrow peak widths, and increased peak capacity compared to larger particle columns. The 2.1 mm ID format significantly reduces solvent consumption while maintaining chromatographic performance, making it an ideal choice for UHPLC, LC-MS compatible methods, and high-throughput analytical laboratories. Because of the low flow rates required, the UHPLC system must be capable of reliable low-flow operation.

This column is especially well suited for HILIC separations of polar and hydrophilic compounds. The unmodified silica surface provides strong retention of polar analytes, making it an excellent choice for the analysis of pharmaceuticals, metabolites, amino acids, carbohydrates, organic acids, nucleotides, and other compounds that are often difficult to retain using reversed-phase chromatography. The combination of ultra-high purity silica and 1.8 µm particle technology delivers outstanding resolution, reproducibility, sensitivity, and peak capacity, making it particularly valuable for advanced method development, impurity profiling, metabolomics, and demanding analytical workflows.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 100Å pore size

• 1.8 µm particle size

• High efficiency separations

• Increased peak capacity

• Excellent for HILIC separations of polar compounds

Typical Applications

• UHPLC analysis

• UPLC-type systems

• HILIC chromatography

• Normal Phase chromatography

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• LC-MS compatible methods

• Method development

• Impurity profiling

Specifications

• Product Type: UHPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 1.8 µm

• Pore Size: 100Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed exclusively for UHPLC and UPLC-type systems

• UPLC is a trademarked brand designation of Waters Corporation

• Excellent for HILIC separations of polar and hydrophilic compounds

• Suitable for Normal Phase chromatography

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• Provides significantly higher efficiency than larger particle columns

• Ideal for reducing run times while maintaining or improving resolution

• Excellent for method development and method optimization

• Reduced solvent consumption and increased sample throughput

• The 2.1 mm ID format is well suited for LC-MS applications

• Requires a UHPLC system capable of reliable low-flow operation and elevated pressures generated by 1.8 µm particles

• Samples should be filtered using a 0.2 µm syringe filter prior to injection

• 150 mm length provides maximum resolving power and peak capacity for complex separations

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