Prep HPLC Column, NP Silica, 5um, 100A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Silica™ Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications involving lipids, polar compounds, and hydrophilic analytes. Manufactured from ultra-high purity, low-metal-content 100 Å silica, this stationary phase is completely unmodified, providing the traditional selectivity associated with USP L3 classified silica. NP Silica™ is widely used when maximum silica activity is desired for normal-phase and HILIC purification methods.
82561-P21-100 is packed with 5 µm NP Silica™ particles in a 21.2 mm ID x 100 mm preparative format. The 5 µm particle size supports preparative flow rates while producing lower backpressure than smaller particle columns. The large internal diameter and column volume provide high sample loading capacity, making this column ideal for purification, fraction collection, and analytical-to-preparative method scale-up.
The 21.2 mm ID x 100 mm format is well suited for preparative HILIC and normal-phase chromatography involving hydrophilic and polar compounds. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative HPLC system capable of the required flow rates and sample loading is necessary for optimum performance.
Key Benefits
NP Silica™ stationary phase
USP L3 equivalent chemistry
Ultra-high purity, low-metal-content silica
Unmodified silica surface
100 Å pore size
Suitable for HILIC applications
Suitable for normal-phase chromatography
High sample loading capacity
Lower backpressure with 5 µm particles
Ideal for preparative purification
Typical Applications
Preparative HILIC chromatography
Preparative normal-phase HPLC
Lipid purification
Polar compound purification
Hydrophilic compound purification
Fraction collection
Method scale-up
Pharmaceutical purification
Process development
USP L3 method transfer
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: NP Silica™
Chromatography Mode: HILIC / Normal Phase
USP Classification: L3
Particle Size: 5 µm
Pore Size: 100 Å
Column Dimensions: 21.2 mm ID x 100 mm
Recommended Flow Rate: 15-25 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Suitable for HILIC applications
Suitable for normal-phase chromatography
Unmodified silica stationary phase
USP L3 equivalent chemistry
Excellent for lipid, hydrophilic, and polar compound purification
Large diameter provides high sample loading capacity
100 mm length offers an excellent balance of throughput and resolution
Typical operating range is approximately 15-25 mL/min
Requires a specialized preparative HPLC system
Ideal for purification, fraction collection, and method scale-up
This is a Special Order Item