Prep HPLC Column, NP Silica, Wide Pore, 5um, 300A. 21.2mm ID x 150mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Silica™ Wide Pore Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications involving larger polar and hydrophilic compounds. Manufactured from ultra-high purity, low-metal-content 300 Å silica, this stationary phase is completely unmodified, providing the traditional selectivity associated with USP L3 classified silica while improving accessibility for higher molecular weight analytes. NP Silica™ Wide Pore is widely used in preparative laboratories and pilot plants for purification of proteins, peptides, lipids, and other large polar compounds.
82561-P21-1503M is packed with 5 µm NP Silica™ Wide Pore particles in a 21.2 mm ID x 150 mm preparative format. The 300 Å pore structure enhances mass transfer and analyte accessibility for larger molecules, while the 5 µm particle size supports preparative flow rates with lower operating backpressures than smaller particles. The increased column length provides enhanced resolution and purification efficiency, while the large internal diameter delivers high sample loading capacity for demanding purification applications.
The 21.2 mm ID x 150 mm format is well suited for preparative HILIC and normal-phase chromatography involving proteins, peptides, lipids, and other large hydrophilic or polar compounds. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative HPLC system capable of the required flow rates and sample loading is necessary for optimum performance.
Key Benefits
NP Silica™ Wide Pore stationary phase
USP L3 equivalent chemistry
Ultra-high purity, low-metal-content silica
Unmodified silica surface
300 Å wide pore structure
Suitable for HILIC applications
Suitable for normal-phase chromatography
Enhanced accessibility for larger molecules
High sample loading capacity
Lower backpressure with 5 µm particles
Typical Applications
Preparative HILIC chromatography
Preparative normal-phase HPLC
Protein purification
Peptide purification
Lipid purification
Biomolecule isolation
Polar compound purification
Fraction collection
Process development
Method scale-up
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: NP Silica™ Wide Pore
Chromatography Mode: HILIC / Normal Phase
USP Classification: L3
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 21.2 mm ID x 150 mm
Recommended Flow Rate: 15-25 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Suitable for HILIC applications
Suitable for normal-phase chromatography
Unmodified silica stationary phase
USP L3 equivalent chemistry
Wide pore design improves accessibility for proteins, peptides, lipids, and larger molecules
Excellent for biomolecule and high molecular weight compound purification
Large diameter provides high sample loading capacity
150 mm length provides increased resolution and purification efficiency
Typical operating range is approximately 15-25 mL/min
Requires a specialized preparative HPLC system
Ideal for purification, fraction collection, and method scale-up
This is a Special Order Item