Catalog Number - 82561-P50-2503M

Cogent NP Silica™ Wide Pore Process HPLC Columns are designed for large-scale purification, process chromatography, and production applications involving larger polar and hydrophilic compounds. Manufactured from ultra-high purity, low-metal-content 300 Å silica, this stationary phase is completely unmodified, providing the traditional selectivity associated with USP L3 classified silica while improving accessibility for higher molecular weight analytes. NP Silica™ Wide Pore is widely used for purification of proteins, peptides, lipids, and other large polar compounds in preparative and process-scale environments.

82561-P50-2503M is packed with 5 µm NP Silica™ Wide Pore particles in a 50 mm ID x 250 mm process-scale format. The 300 Å pore structure enhances mass transfer and analyte accessibility for larger molecules, while the 5 µm particle size supports high process flow rates with lower operating backpressures than smaller particles. The extended column length provides maximum resolution and purification efficiency, while the large internal diameter delivers extremely high sample loading capacity for demanding production-scale purification applications.

The 50 mm ID x 250 mm format is well suited for process HILIC and normal-phase chromatography involving proteins, peptides, lipids, and other large hydrophilic or polar compounds. Typical flow rates are approximately 80-140 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative or process HPLC system capable of high flow rates and large-volume sample loading is required for optimum performance.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity, low-metal-content silica

• Unmodified silica surface

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Enhanced accessibility for larger molecules

• Extremely high sample loading capacity

• Lower backpressure with 5 µm particles

Typical Applications

• Process HILIC chromatography

• Process normal-phase HPLC

• Protein purification

• Peptide purification

• Lipid purification

• Biomolecule isolation

• Polar compound purification

• Production-scale separations

• Process development

• Method scale-up

Specifications

• Product Type: Process HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• USP Classification: L3

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 50 mm ID x 250 mm

• Recommended Flow Rate: 80-140 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Unmodified silica stationary phase

• USP L3 equivalent chemistry

• Wide pore design improves accessibility for proteins, peptides, lipids, and larger molecules

• Excellent for biomolecule and high molecular weight compound purification

• Large 50 mm ID provides extremely high sample loading capacity

• 250 mm length provides maximum resolution and peak capacity

• Typical operating range is approximately 80-140 mL/min

• Requires a specialized preparative or process HPLC system capable of high flow rates

• Ideal for production-scale purification, fraction collection, and process development

• This is a Special Order Item