HPLC Column, RP C18 PE, 3um, 2.1mm ID x 50mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 84318‑05P‑2 Cogent RP C18 PE™ HPLC Column is engineered for enhanced retention of polar analytes while maintaining traditional reversed‑phase behavior, utilizing 3 µm particles and a polar embedded amide ligand within a C18 chain.
This stationary phase is manufactured using high‑purity silica with fully double end‑capped bonding, providing:
Reduced silanol interactions
Improved peak shape for polar and basic analytes
Stable retention under high aqueous mobile phase conditions (including 100% water)
The embedded amide functional group allows greater interaction with polar compounds compared to traditional C18 phases, while retaining hydrophobic selectivity for non‑polar analytes.
50 mm Length – Fast LC‑MS & High‑Throughput Analysis
Designed for rapid separations and short run times
Ideal for high‑throughput analytical laboratories
Minimizes solvent consumption
Frequently used in LC‑MS workflows
2.1 mm ID – LC‑MS / Low‑Flow Optimization
Optimized for low‑flow HPLC and LC‑MS systems
Enhances MS sensitivity due to reduced solvent load
Ideal for:
High‑sensitivity detection
mass spectrometry coupling
Typical Flow Rate Range
~0.2–0.3 mL/min
✅ Requires HPLC or UHPLC systems capable of stable low‑flow performance
3 µm Particle – High Efficiency Performance
Higher efficiency than 5 µm columns of the same phase
Produces sharper peaks and improved resolution
Ideal for analytical method development and LC‑MS analysis
Why Choose RP C18 PE™ (Polar Embedded)
Enhanced Polar Retention
Retains polar compounds without phase collapse
Stable in 100% aqueous mobile phases
Improves retention of hydrophilic analytes compared to standard C18
Dual‑Mode Capability
Functions in:
Reversed Phase (RP)
HILIC‑like behavior under appropriate conditions
Ideal for Challenging Methods
Suitable for:
Polar analytes with poor retention on standard C18
complex mixtures with wide polarity range
Excellent for method development flexibility
USP Method Compatibility
Suitable as a USP L1 alternate
Ideal for methods requiring:
high aqueous mobile phases
polar compound retention
Typical Applications
LC‑MS analysis
Polar and hydrophilic compound separations
Pharmaceutical method development
Metabolite analysis
Peptide and small molecule analysis
Long‑chain fatty acids
Cogent RP C18 PE™ Advantages
Polar Embedded Ligand
Enhances interaction with polar analytes
Improves retention without sacrificing hydrophobic selectivity
Fully End‑Capped Surface
Reduces secondary interactions
Improves peak symmetry and reproducibility
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs
Important Usage Notes
Optimized for 2.1 mm ID LC‑MS and low‑flow applications
Ideal for high aqueous mobile phases (including 100% water)
Suitable for both RP and mixed‑mode separations
Ensure system capability for stable low‑flow delivery