Catalog Number - 84318-10P

The 84318‑10P Cogent RP C18 PE™ HPLC Column is engineered to provide enhanced retention of polar analytes while maintaining traditional reversed‑phase selectivity, using a polar embedded amide group within a C18 ligand and 3 µm high‑efficiency particles.

Manufactured on high‑purity silica with fully double end‑capped bonding, this phase delivers:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phase conditions, including 100% water

The embedded amide functionality increases interaction with polar compounds, preventing the phase collapse often seen with conventional C18 columns while maintaining strong hydrophobic retention.

100 mm Length – Increased Resolution for Analytical Development

• Higher resolving power than 50 mm columns

• Improved separation of closely related compounds

• Increased peak capacity for analytical workflows

• Ideal for method development and refinement

This configuration is selected when greater resolution is required beyond fast screening methods.

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • QC and routine analysis

• Provides robust and reproducible performance

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical HPLC systems without special flow requirements

3 µm Particle – High Efficiency Performance

• Higher efficiency than 5 µm columns of the same phase

• Produces sharper peaks and improved resolution

• Ideal for analytical precision and optimized separations

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Functions in:

  • Reversed Phase (RP)

  • HILIC‑like modes under appropriate conditions

Ideal for Challenging Samples

• Suitable for:

  • polar analytes poorly retained on conventional C18

  • mixed polarity compounds

• Enables flexible method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • robust retention of polar analytes

Typical Applications

• Analytical method development

• Polar and hydrophilic compound separations

• Pharmaceutical QC and testing

• Mixed polarity sample analysis

• Long‑chain fatty acids

• Method refinement prior to LC‑MS or prep scaling

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains classic C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable run‑to‑run reproducibility

Important Usage Notes

• Ideal for analytical HPLC method development and refinement

• Suitable for high aqueous mobile phases (including 100% water)

• Compatible with standard analytical systems (4.6 mm ID)

• Optimize depending on RP or mixed‑mode operation