Catalog Number - 84518-10P

The 84518‑10P Cogent RP C18 PE™ HPLC Column is engineered for robust analytical separations with improved polar retention, utilizing 5 µm particles and a polar embedded amide group within a C18 ligand to deliver stable chromatographic performance across a wide range of analytes.

Manufactured using high‑purity silica with fully double end‑capped bonding, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for polar and basic analytes

• Stable retention under high aqueous mobile phases, including 100% water

The embedded amide functionality allows enhanced interaction with polar compounds, preventing phase collapse associated with traditional C18 columns, while maintaining strong hydrophobic retention for non‑polar analytes.

100 mm Length – Increased Resolution for Analytical Development

• Higher resolving power than 50 mm columns

• Improved separation of closely related compounds

• Increased peak capacity for analytical workflows

• Ideal for method development and refinement

This configuration is selected when greater resolution is required beyond fast screening methods.

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • quality control (QC)

  • routine and regulated workflows

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical systems without special flow requirements

5 µm Particle – Rugged & Reproducible Performance

• Designed for durability and long column lifetime

• Provides reliable performance across routine analytical workflows

• Ideal for method transfer and reproducibility across systems

✅ Preferred when robustness and stability are prioritized over maximum efficiency

Why Choose RP C18 PE™ (Polar Embedded)

Enhanced Polar Retention

• Retains polar compounds without phase collapse

• Stable in 100% aqueous mobile phases

• Improves retention compared to standard C18 columns

Dual‑Mode Flexibility

• Operates in:

  • Reversed Phase (RP)

  • HILIC‑like modes under appropriate conditions

Ideal for Mixed Polarity Samples

• Suitable for:

  • polar analytes poorly retained on conventional C18

  • complex mixtures with broad polarity ranges

• Enables robust and flexible method development

USP Method Compatibility

• Suitable as a USP L1 alternate

• Ideal for methods requiring:

  • high aqueous mobile phases

  • consistent retention of polar analytes

Typical Applications

• Analytical method development

• Pharmaceutical QC and testing

• Polar and hydrophilic compound separations

• Mixed polarity sample analysis

• Long‑chain fatty acids

• Method refinement workflows

Cogent RP C18 PE™ Advantages

Polar Embedded Ligand

• Enhances interaction with polar analytes

• Maintains classic C18 hydrophobic selectivity

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry and reproducibility

High Purity Silica

• Consistent chromatographic performance

• Reliable batch‑to‑batch reproducibility

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Ideal for method development and increased‑resolution workflows

• Suitable for high aqueous mobile phases (including 100% water)

• Optimize depending on RP or mixed‑mode separation conditions