Catalog Number - 62508-05P-2

Hyper Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octyl (C8) bonded phase. Designed as a cost-effective alternative to legacy Hypersil® and Spherisorb® columns, it offers comparable chromatographic behavior for USP L7 methods while providing the moderate hydrophobic retention and reproducibility expected from a conventional C8 stationary phase. Compared to C18 columns, C8 phases generally provide reduced retention and faster elution of hydrophobic analytes.

62508-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 5 µm, 150Å Hyper Similars C8 particles. The short 50 mm length is optimized for rapid analyses and reduced solvent consumption, while the 2.1 mm ID format is particularly well suited for LC-MS applications. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of precise low-flow delivery with appropriately sized flow cells and low-volume plumbing. The 5 µm particle size offers reliable performance with moderate operating pressure.

This USP L7 column is ideal for hydrophobic and neutral compounds when less retention is desired than a comparable C18 phase. Its combination of low-flow operation, fast run times, and traditional C8 selectivity makes it a popular choice for LC-MS methods, pharmaceutical analysis, and long-chain fatty acid applications.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Hypersil® and Spherisorb® C8 columns

• Narrow-bore format ideal for LC-MS applications

• Fast analysis with 50 mm column length

• Lower retention than C18 columns

• Reduced solvent consumption

• End-capped C8 bonded phase

Typical Applications

• USP L7 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Long-chain fatty acid analysis

• Fast analytical separations

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C8 Octyl

• USP Classification: L7

• Particle Size: 5 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Lower operating pressure than comparable 3 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Provides less retention than comparable C18 stationary phases

• Excellent for high-throughput LC-MS workflows

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