Syringe Filters, 4mm, PES, 0.45um Pore Size. Black Polypropylene, AQ Brand. 100/PK.
Additional Info:
The 58045-PES04-C AQ™ Syringe Filters are pharmaceutical-grade, ultra-low volume membrane filters engineered for HPLC sample preparation and dissolution testing in regulated laboratory environments. Featuring a 0.45 µm Polyethersulfone (PES) membrane, these filters deliver exceptional throughput, ultra-low protein binding, and extractable-free performance, ensuring accurate and reproducible analytical results—even with extremely limited sample volumes.
The 4 mm format is specifically designed for micro-volume filtration, minimizing hold-up volume and maximizing recovery from high-value protein and biological samples, making these filters ideal for LC‑MS workflows and precision analytical applications.
✅ Critical Compliance Advantage (No Flush Required)
These filters are validated and certified to meet stringent regulatory requirements, including:
US FDA CFR Title 21, Part 210.3(b)(6) for minimum fiber release
USP <788> for particulate matter in injectables
USP requirements for “Sterile Water for Injection”
Because of this validated performance:
✅ No initial flushing is required
✅ No need to discard the first 20 µL of filtrate
✅ Preserves extremely valuable micro-volume samples
✅ Saves time and reduces risk of failed methods and investigations
👉 This delivers a critical operational advantage for QC/QA and biopharma laboratories, where every microliter matters and protein recovery is essential.
Performance & Design
The 0.45 µm pore size is optimized for sample clarification and prefiltration, delivering high flow rates and reduced clogging, even in ultra-small format filters.
PES membrane chemistry provides ultra-low protein binding, low extractables, and excellent flow characteristics, ensuring maximum recovery of proteins and biomolecules, even from limited sample volumes.
The 4 mm design minimizes hold-up volume, making these filters ideal for LC‑MS sample preparation, method development, and micro-volume analytical workflows.
The black housing provides quick identification of 0.45 µm PES filters, and each pack includes a Certificate of Analysis for full traceability and regulatory compliance.
Key Features
0.45 µm PES Membrane (Pharma Grade)
Ideal for clarification and prefiltration
High flow and efficient performance
✅ No Initial Flush Required (Validated)
Extractable-free from first filtrate
No 20 µL discard required
Ultra-Low Protein Binding
Maximizes recovery of proteins and biomolecules
Ideal for biological and biopharma samples
Ultra-Low Volume Format (4 mm)
Minimizes hold-up volume
Maximizes recovery from micro-volume samples
High Throughput Performance
Fast filtration with minimal resistance
Ideal for analytical workflows
Low Extractables
Ensures clean chromatography
Improves reproducibility
Regulatory Compliance
Meets FDA CFR Title 21 requirements
Complies with USP <788>
Suitable for Sterile Water for Injection
Autoclavable & EO Sterilizable
121°C for 30 minutes or Ethylene Oxide
Color-Coded (Black)
Identifies 0.45 µm PES filters
Typical Applications
HPLC & UHPLC Sample Preparation – micro-volume sample clarification
LC‑MS Workflows – maximum recovery with minimal loss
Protein & Biomolecule Filtration – low binding, high recovery
Cell Culture Media & Buffers – clean filtration
Method Development – limited or high-value samples
Dissolution Testing – pharmaceutical QC workflows
Regulated Laboratory Methods – validated, compliant processes
Specifications
Filter Type: Syringe Filter (Pharma Grade)
Membrane Material: PES (Polyethersulfone)
Pore Size: 0.45 µm
Diameter: 4 mm
Housing Material: Polypropylene (PP)
Color Code: Black (0.45 µm PES)
Connection Type: Luer Lock / Slip
Chemical Compatibility: Aqueous solutions, buffers, biological samples
Protein Binding: Ultra-low
Extractables: None detectable (no flush required)
Initial Discard Volume: None (20 µL not required)
Sterilization: Autoclave or EO
Compliance: FDA CFR 21, USP <788>
Certification: Certificate of Analysis included
Sterility: Non-sterile
Pack Size: 100/PK
Brand: AQ™
Resources & Ordering Options