Catalog Number - 75040-10P

Cogent HPS Amino™ HPLC Columns are designed for the analysis of highly polar compounds using Normal Phase and HILIC chromatography modes. Manufactured using tri-functional bonding of a three-carbon silane with a terminal amino functional group bonded to high-purity silica, this stationary phase is not end capped and provides the selectivity required for challenging separations of carbohydrates, sugars, organic acids, and other polar analytes. HPS Amino™ is widely used as an alternative for USP L8 methods and for new method development requiring amino phase selectivity.

75040-10P is packed with 5 µm HPS Amino™ particles in a 4.6 mm ID x 100 mm analytical format. The 100 mm length provides an excellent balance of chromatographic resolution and analysis speed, making it well suited for routine laboratory use, method development, and quality control applications. The 5 µm particle size offers reliable efficiency with lower operating backpressure compared to smaller particle columns, making it compatible with conventional HPLC systems.

The HPS Amino™ stationary phase is particularly effective for negatively charged polar compounds, including sugars, carbohydrates, and compounds containing carboxylic acid functional groups. Commonly used in both Normal Phase and HILIC chromatography, this column provides excellent retention and selectivity for analytes that can be difficult to retain on traditional reversed-phase columns. Its versatile performance makes it suitable for food analysis, pharmaceutical testing, and general analytical separations involving polar compounds.

Key Benefits

• HPS Amino™ stationary phase

• USP L8 equivalent chemistry

• Tri-functional amino bonding

• High-purity silica support

• 5 µm particle size

• Suitable for HILIC separations

• Suitable for Normal Phase chromatography

• Lower operating backpressure

• Excellent for sugars and organic acids

• Balanced speed and resolution

Typical Applications

• USP L8 methods

• HILIC chromatography

• Normal Phase HPLC

• Sugar analysis

• Carbohydrate analysis

• Organic acid analysis

• Polar compound separations

• Food and beverage testing

• Method development

• Quality control testing

Specifications

• Product Type: Analytical HPLC Column

• Stationary Phase: HPS Amino™

• USP Classification: L8

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 5 µm

• Pore Size: 120Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L8 equivalent chemistry

• Not end capped

• Designed for polar and negatively charged compounds

• Excellent for sugars and compounds containing carboxylic acid groups

• Compatible with standard analytical HPLC systems

• Frequently compared with Inertsil™ Amino and Luna™ Amino columns

• Lower backpressure than smaller particle columns

• 100 mm length provides an excellent balance of speed and resolution

• Suitable for routine analytical methods and method development

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