Guard Column Starter Set, Amide, complete with 5 each 4.0 mm ID x 10 mm long cartridges packed with 4 um, 100 A phase and 1 each guard column holder. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑PG4 Amide Guard Column Starter Set provides everything required to implement Cogent TYPE‑C™ guard column protection for larger‑ID Amide HILIC analytical columns. This complete starter kit is designed to protect analytical columns from particulates, strongly retained contaminants, and irreversible matrix components that can degrade chromatographic performance and shorten column life.
The kit includes a stainless‑steel guard cartridge column holder compatible with TYPE‑C™ guard cartridges, along with five Amide guard cartridges packed with 4 µm, 100 Å Amide stationary phase.
Guard Column Use Case: Amide HILIC Protection for Larger‑ID Analytical Columns
Guard columns act as a sacrificial protection stage installed upstream of the analytical column. When running HILIC methods using Amide chemistry on 3.0–4.6 mm ID analytical columns, guard columns help:
Protect analytical columns from particulate contamination
Trap strongly retained or irreversible sample components
Preserve chromatographic selectivity, peak shape, and retention stability
Extend analytical column lifetime and reduce replacement frequency
Guard protection is strongly recommended for polar, matrix‑rich samples, including food, biological, metabolomics, and pharmaceutical HILIC workflows.
What’s Included in the Starter Set
This guard column starter kit includes:
5 Amide guard cartridges, each 4.0 mm ID × 10 mm length, packed with 4 µm, 100 Å Amide phase
Based on average analytical use, the five included cartridges typically provide sufficient protection for the lifetime of one analytical column.
Each guard cartridge is shipped in an individual, closeable Delrin® storage case to prevent drying, contamination, or handling damage prior to installation.
Phase Consistency: Amide TYPE‑C™ Chemistry
The guard cartridges in this kit are packed with the same Amide TYPE‑C™ silica hydride chemistry used in corresponding analytical columns, ensuring:
No change in chromatographic selectivity or elution order
Minimal added dead volume
Seamless integration into existing Amide HILIC methods
Stable performance under high‑organic HILIC conditions
Maintaining phase consistency is essential to preserving method integrity when guard protection is implemented.
Compatibility & Recommended Use
Designed for 3.0 mm, 4.0 mm, and 4.6 mm ID analytical HPLC columns
Compatible with Cogent TYPE‑C™ Amide analytical columns
Suitable for HILIC workflows targeting sugars, amines, and highly polar analytes
This starter set is ideal for laboratories implementing guard protection on larger‑diameter analytical columns or standardizing Amide guard usage across multiple methods.
Operational Notes
Guard cartridges can be replaced quickly without removing the analytical column
Replacement frequency depends on sample cleanliness and matrix complexity
Guard use is strongly recommended for LC‑MS, food, metabolomics, biological, and other complex polar samples