Catalog Number - 40231-PG1

40231-PG1 Cogent UDA™ 2.2 µm (WCX) Guard Column Starter Set provides a complete, high‑performance solution for protecting analytical columns used in UHPLC and LC‑MS workflows while maintaining the unique mixed‑mode selectivity of UDA™ chemistry.

This kit includes a high‑pressure capable Cogent Guard Column Holder and five guard column cartridges, each packed with 2.2 µm, 120 Å Cogent UDA™ TYPE‑C™ silica. The smaller particle size ensures high efficiency and low dispersion, making this kit ideal for modern UHPLC systems.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This matched mixed‑mode chemistry ensures contaminants are captured without compromising chromatographic selectivity, preserving method accuracy and reproducibility.

Each cartridge is individually packaged in resealable Delrin cases, preventing drying and contamination prior to use.

2.1 mm ID Compatibility – UHPLC & LC‑MS Protection

• Designed for 2.1 mm ID analytical columns

• Minimizes extra-column dispersion in low‑volume systems

• Protects against particulates and strongly retained contaminants

• Maintains resolution, sensitivity, and peak shape

✅ Recommended for UHPLC and LC‑MS methods requiring maximum efficiency and low dispersion

Flow Rate & System Requirements (2.1 mm ID, 2.2 µm)

⚠️ Requires UHPLC or low‑flow capable HPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Higher backpressure due to 2.2 µm particles

• Designed for low‑volume, high‑pressure systems

• Compatible with MS and UV detectors

✅ Important: Systems must be capable of stable, precise low‑flow delivery and elevated pressure operation

✅ Best Practice: Replace guard cartridges regularly to maintain system performance and protect analytical columns

Why Use UDA™ Guard Columns (2.2 µm)

Maximum Column Protection

• Prevents contamination from particulates and matrix components

• Reduces irreversible adsorption on analytical columns

Maintains High‑Efficiency Separations

• Matches UDA™ RP, WCX, and HILIC selectivity

• Preserves resolution in UHPLC methods

Supports LC‑MS Performance

• Maintains sensitivity and peak integrity

• Reduces method variability

TYPE‑C Silica Advantages

Faster Equilibration

• Reduces downtime between runs

No Strict Moisture Control (HILIC)

• Simplifies mobile phase preparation

Extreme Robustness

• Stable at low pH (<2)

• Resistant to hydrolysis

Long Cartridge Life

• Durable in demanding UHPLC workflows

Cogent UDA™ Guard Kit Advantages

Complete High‑Performance Starter Solution

• Includes high‑pressure holder and cartridges

Matched Mixed‑Mode Chemistry

• Seamless integration with UDA™ analytical columns

Optimized for UHPLC Systems

• Low dispersion with high efficiency

Convenient Cartridge Storage

• Individual protective cases preserve performance

Important Usage Notes

✅ Included Hardware: This starter set includes a Cogent Guard Column Holder designed for high‑pressure UHPLC operation, compatible with all Cogent guard cartridges (view Cogent Guard Column Holder specifications and compatibility details)

• Designed for 2.1 mm ID analytical UHPLC columns

• Replace cartridges when system pressure increases or performance declines

• Store unused cartridges in protective cases