Catalog Number - 40231-PG2

40231-PG2 Cogent UDA™ (WCX) Guard Column Cartridges are designed to protect high‑efficiency analytical columns from particulates and strongly retained contaminants that can degrade performance and reduce column life.

Each cartridge is packed with 2.2 µm, 120 Å Cogent UDA™ stationary phase, built on TYPE‑C™ silica (silica hydride platform). The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This matched mixed‑mode chemistry ensures contaminants are trapped effectively while maintaining identical selectivity to UDA™ analytical columns, preserving method accuracy and reproducibility.

Each cartridge is constructed in a stainless steel tube with stainless steel frits, labeled for traceability, and individually packaged in protective Delrin cases to prevent drying, contamination, and handling damage.

2.0 mm ID × 10 mm Length – UHPLC & LC‑MS Protection

• Designed for 2.1 mm ID analytical columns

• Minimizes dispersion in low‑volume UHPLC systems

• Protects against particulates and matrix contaminants

• Maintains peak shape, sensitivity, and resolution

✅ Recommended for UHPLC and LC‑MS workflows requiring high efficiency and low dispersion

Flow Rate & System Requirements (2.1 mm ID, 2.2 µm)

⚠️ Requires UHPLC or low‑flow capable HPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Higher backpressure expected with 2.2 µm particles

• Designed for high‑pressure, low‑volume systems

• Compatible with MS and UV detectors

✅ Important: Systems must be capable of accurate low‑flow delivery and high‑pressure operation

✅ Best Practice: Replace cartridges regularly to maintain performance and protect analytical columns

Why Use UDA™ 2.2 µm Guard Cartridges

Column Protection

• Prevents particulate contamination and matrix buildup

• Reduces irreversible adsorption to analytical columns

Maintains High‑Efficiency Separations

• Preserves RP, WCX, and HILIC selectivity

• Maintains resolution and method sensitivity

Supports LC‑MS Workflows

• Reduces variability

• Maintains consistent peak integrity

TYPE‑C Silica Advantages

Faster Equilibration

• Reduces downtime between runs

No Strict Moisture Control (HILIC)

• Simplifies solvent handling

Extreme Robustness

• Stable under low pH conditions (<2)

• Resistant to hydrolysis

Durable Cartridge Design

• Long life in demanding UHPLC workflows

Cogent UDA™ Guard Cartridge Advantages

Matched Mixed‑Mode Chemistry

• Seamless compatibility with UDA™ analytical columns

High‑Efficiency Design (2.2 µm)

• Maintains UHPLC performance

Low‑Dispersion Construction

• Optimized for LC‑MS systems

Individual Protective Packaging

• Prevents drying and contamination

Typical Applications

• UHPLC and LC‑MS workflows

• Complex matrix separations

• Method development and validation

• Polar and ionizable compound analysis

• Pharmaceutical and bioanalytical testing

Important Usage Notes

✅ Required Hardware: These guard column cartridges must be used with a Cogent Guard Column Holder (view Cogent Guard Column Holder specifications and compatibility information)

• Designed for 2.1 mm ID analytical columns

• Packed and shipped in reversed‑phase solvents

• Replace cartridges when backpressure increases or performance declines

• Store unused cartridges in protective cases