Anti- A1- Glycoprotein - Monoclonal Protein - AppNote
May 9, 2012
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Date: 9-MAY-2012 Last Updated: 31-AUGUST-2026
Glycoprotein Analysis
For the last few decades Glycoproteins have been a subject of interest to biochemists and biologists in many fields. These proteins are found in plasma and other biological fluids and they serve many functions in nearly every physiological process of living organisms.These macromolecules consist of a peptide chain and one or more carbohydrates linked to them. The simple gradient Method used in this note for the analysis is very reproducible (%RSD about 1.5). The equilibration time between samples is very short and the symmetrical peak is easy to integrate.
Method Conditions
Column: Cogent Bidentate C8 300™, 5 μm, 300 Å
Catalog No.: 40008-75P-3M
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- A: DI Water / 0.1% Trifluoroacetic Acid
-- B: Acetonitrile / 0.1% Trifluoroacetic Acid
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 15 |
| 20 | 50 |
| 25 | 50 |
| 25.1 | 15 |
Injection vol.: 1 μL
Flow rate: 0.5 mL / minutes
Detection: UV @ 214 nm
Sample Preparation: Monoclonal Anti-A1-Glycoprotein, Mouse 043H4848 in 0.05 % (w/v) in DI Water.
Notes: This Glycoprotein is associated with inflammatory response and its related conditions such as Rheumatoid Arthritis.
