4-Aminoacetophenone Analysis with HPLC - AppNote
May 14, 2012
/
/
/
Date: 14-MAY-2012 Last Updated: 31-AUGUST-2026
4’-Aminoacetophenone Retained in Acidic Conditions
This Method investigated 4’-Acetaminophenone Retention and Analysis. Adequate Retention and Sensitivity was the result and could be useful in Analysis of the Metabolites of this class of Anti-tumor Agents in body fluids (Plasma or Urine) during or after Chemotherapy.
Peak: 4’-Aminoacetophenone
Method ConditionsColumn: Cogent Bidentate C18™, 4 μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- Isocratic: 80:20 Solvent A / Solvent B
-- A: DI Water / 0.2% Acetic Acid
-- B: Acetonitrile / 0.2% Acetic Acid
Injection vol.: 2 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 324 nm
Sample Preparation: 1 mg of the Compound dissolved in 1 mL of 50:50 Solvent A / Solvent B solution.
Sample for Injection diluted 1:15 with 100% Solvent A.
Note: 4’-Aminoacetohenone (arylamine) is one of the metabolites of 1-(4-Acetylphenyl)-3,3-Dimethyltriazene, which is an anti-tumour Triazene. 4’-Acetaminophenone is also frequently used as an internal standard in analysis of Mitomycin C.
