Organic Alcohol Separation by HPLC Analysis of 1-Phenyl-2-Propanol and 2-Phenyl-1,2-Propanediol - AppNote
November 28, 2012
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Date: 28-NOVEMBER-2012 Last Updated: 30-AUGUST-2026
Separation of 1-Phenyl-2-Propanol and 2-Phenyl-1,2-Propanediol
In this Method, Separation is obtained between two structurally similar Organic Alcohols. The Method illustrates the type of Retention and Selectivity that can be obtained for these kinds of compounds. Furthermore, the Mobile Phase Solvents are simple to prepare and LCMS compatible.The overlay of the 5 Figures demonstrates how the Method is Precise and Reliable when the Column is changed with lot-to-lot, which is a critical feature. RSD values of 0.5% and 0.3% were obtained for the two Peaks between the 5 lots.
1. 1-Phenyl-2-Propanol 2. 2-Phenyl-1,2-Propanediol
Column: Cogent Bidentate C18™, 4 µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- A: DI Water / 0 .1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 10 |
| 2 | 10 |
| 6 | 50 |
| 7 | 10 |
Flow rate: 1.0ml / minute
Detection: UV @ 220 nm
Post time: 3 minutes
Sample Preparation: 0.1µL / mL 1-Phenyl-2-Propanol and 0.1mg / mL 2-Phenyl-1,2-Propanediol in Diluent of 50:50 Solvent A / Solvent B.
t 0 : 0.9 minutes
Note: These two compounds are used as starting materials in organic syntheses. As such, the Method has relevance in impurities testing of finished products to ensure no starting materials are present.
