MDMA Analysis in Plasma Samples with LCMS - AppNote
September 27, 2013
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Date: 27-SEPTEMBER-2013 Last Update: 14-AUGUST-2026
Under the described conditions, MDMA was retained and eluted as a Symmetrical Peak. The Sensitivity of the Method is very good and comparable to that reported with GCMS Detection
[1]
. Matrix effects were of minor extent and reproducible and hence should not compromise Quantification. The Method can be used for Forensic Research and Clinical Analysis.
Post Time: 3 minutes
Flow rate: 0.4 mL / minute
Injection vol.: 1 μL
Analysis of MDMA in Plasma Samples with LCMS pdf 0.2 Mb Download File
Methylenedioxymethamphetamine Analyzed with LC-MS
Cogent™ Phenyl Hydride Column Ordering Information
Peak:
(±)-3,4-Methylenedioxymethamphetamine, m/z 194.1176 [M+H]+
(±)-3,4-Methylenedioxymethamphetamine, m/z 194.1176 [M+H]+
Method Conditions
Column: Cogent Phenyl Hydride™, 4 μm, 100 Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
-- A: DI Water / 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
Column: Cogent Phenyl Hydride™, 4 μm, 100 Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
-- A: DI Water / 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 10 |
| 1 | 10 |
| 6 | 90 |
| 7 | 10 |
Post Time: 3 minutes
Flow rate: 0.4 mL / minute
Injection vol.: 1 μL
Sample Preparation: 50 μl of Acetonitrile was mixed with 50μl of plasma for protein precipitation. The samples were centrifuged
(16000×g for 15 minutes)
, and the supernatant was filtered through a 0.45 μm Nylon Syringe Filter (MICROSOLV Tech Corp.) and transferred to autosampler vials for injection.
Detection: ESI – POS - Agilent 6210 MSD TOF Mass Spectrometer
t 0 : 0.9 minutes
Detection: ESI – POS - Agilent 6210 MSD TOF Mass Spectrometer
t 0 : 0.9 minutes
Note: The Amphetamine derivative 3,4-methylenedioxymethamphetamine (MDMA), known also as Molly or Ecstasy, is often used or abused as a recreational drug. Because of a reported high inter-individual difference of its toxicity, sensitive analytical methods are needed. A urine test is a standard method to investigate drug abuse but the method has a very low diagnostic sensitivity and makes testing in plasma much more suitable.
Reference: [1]. R. Kikura, Y. Nakahara, T. Mieczkowski, F. Tagliaro, Forensic Sci. Int. 84 (1997) 165–177.
Reference: [1]. R. Kikura, Y. Nakahara, T. Mieczkowski, F. Tagliaro, Forensic Sci. Int. 84 (1997) 165–177.
Analysis of MDMA in Plasma Samples with LCMS pdf 0.2 Mb Download File